Anti mis12

Manufactured by Abcam

Anti-MIS12 is a primary antibody that recognizes the MIS12 protein. MIS12 is a component of the kinetochore-associated MIS12 complex, which plays a crucial role in chromosome segregation during cell division. The Anti-MIS12 antibody can be used to detect and study the MIS12 protein in various applications, such as Western blotting, immunoprecipitation, and immunofluorescence.

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Lab products found in correlation

Anti β tubulin, by Santa Cruz Biotechnology (1 mentions) Anti mettl3, by Proteintech (1 mentions) Anti mettl14, by Merck Group (1 mentions) Alexa fluor 488 donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 568 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti m6a, by Synaptic Systems (1 mentions) Mouse monoclonal anti hec1, by Abcam (1 mentions) Mouse monoclonal anti acetylated tubulin, by Merck Group (1 mentions) Anti aurora b, by Abcam (1 mentions)

2 protocols using anti mis12

Antibodies for m6A Detection and Analysis

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Antibodies used for western blotting, dot blotting, immunostaining or RNA immunoprecipitation (RIP): anti-m⁶A (Synaptic Systems, 202003), anti-METTL3 (Proteintech, 15073-1-AP), anti-METTL14 (Sigma, HPA038002), anti-MIS12 (Abcam, ab70843), anti-IGF2BP2 (Bethyl Laboratories, A303–317A), anti-β-Tubulin (Santa Cruz, sc-5274), anti-GAPDH (Santa Cruz, sc-25778), anti-β-Actin (Santa Cruz, sc-69879), horseradish peroxidase (HRP)-conjugated secondary antibodies (ZSGB-BIO, ZB2305 and ZB2301), Alexa Fluor 488 donkey anti-mouse IgG (Invitrogen, A21202), Alexa Fluor 568 donkey anti-rabbit IgG (Invitrogen, A10042).

Wu Z., Shi Y., Lu M., Song M., Yu Z., Wang J., Wang S., Ren J., Yang Y.G., Liu G.H., Zhang W., Ci W, & Qu J. (2020). METTL3 counteracts premature aging via m6A-dependent stabilization of MIS12 mRNA. Nucleic Acids Research, 48(19), 11083-11096.

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Indirect Immunostaining of Cytoskeletal and Chromosomal Proteins

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The following primary antibodies were used for indirect immunostaining: mouse monoclonal anti-α-tubulin (1:1,000; Calbiochem), mouse monoclonal anti-acetylated tubulin (1:1,000; Sigma-aldrich), rabbit polyclonal anti-CENP-B (1:1,000; Millipore), rabbit polyclonal anti-histone H3K9me3 (1:2,500; Millipore), mouse monoclonal anti-HP1α (1:400; Millipore), rabbit polyclonal anti-Aurora B (1:500; Abcam), mouse monoclonal anti-HEC1 (1:200; Abcam), rabbit polyclonal anti-Mis12 (1:200; Abcam), mouse monoclonal anti-CENP-F (1:200; Abcam), and human anti-centromere autoantibody (CREST, 1:1,000; Cortex Biochem).

Phengchat R., Takata H., Uchiyama S, & Fukui K. (2017). Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore. Scientific Reports, 7, 7335.

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