1 butanol 1 but

RPMI-1640 cell culture medium, penicillin/streptomycin and L-glutamine were purchased from Lonza (Walkerville, IL, USA). Fetal bovine serum (FBS) was ordered from Atlanta Biologicals (Norcross, GA, USA). MaR2 and MaR1 were purchased from Cayman Chemical (Ann Arbor, MI, USA), stored in an ethanol solution at −80 °C. The solution was diluted immediately before use in Krebs-Ringer bicarbonate buffer with HEPES (KRB-HEPES, 119 mM NaCl, 4.8 mM KCl, 1.0 mM CaCl₂, 1.2 mM MgSO₄, 1.2 mM KH₂PO₄, 25 mM NaHCO₃, 10 mM HEPES, and 5.5 mM glucose (pH 7.40–7.45)) to the desired concentrations and added to the cells. UEA-1 was obtained from Sigma-Aldrich (St. Louis, MO, USA). Vasoactive intestinal peptide (VIP), U73122 and U73343 were purchased from Tocris Bioscience (Ellisville, MO, USA). Histamine, carbachol (CCh), 2APB, 1-butanol (1-but) and tert-butanol (t-but) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Fura-2/AM was purchased from Life Technologies (Grand Island, NY, USA). n-BOC-Phe-Leu-Phe-Leu-Phe (BOC2) was ordered from GenScript (Piscatawy, NJ, USA). BLT1 inhibitor U-75302 and LTB₄ were ordered from Cayman Chemical (Ann Arbor, MI, USA). Lipoxin A₄, H89, thapsigargin and RO-317549 were ordered from EMD Millipore (Billerica, MA, USA).