Huaecs

HUVECs were purchased from Gibco. HUAECs were from Provitro. HUVECs and HUAECs were cultured in EGM-2 medium (CC-3156, Lonza) containing supplements (CC-4176 or CC-4147), respectively, and were used for a maximal number of 6 passages. All cells were cultured at 37 °C and 5% CO₂ and were tested negative for mycoplasma contamination before experiments.

Culturing of ECs in vitro. Culturing of HUVECs and HUAECs (Lonza, Basel, Switzerland) was done according to the manufacturer's protocol.

HUVECs and human umbilical artery endothelial cells (HUAECs) were purchased from Lonza and cultured in EC medium ECM (ScienCell) supplemented with 5% fetal calf serum (FCS, ScienCell), 1% penicillin/streptomycin (ScienCell) and endothelial growth supplement (ScienCell) or endothelial basal medium (EBM; Lonza) supplemented with EGM SingleQuots (Lonza), and 10% FCS (Invitrogen). Microvascular endothelial cells were isolated from pleura-free peripheral lung tissues and pulmonary artery endothelial cells from rings of the arteria pulmonalis, as described previously^{48 (link)} and cultured in ECM. Cells were used for experiments until passage four. Cell numbers were determined by an automated cell counter (Countess II FL, Invitrogen).
HEK293T cells were purchased from ATCC and cultured in Dulbecco’s modified Eagle medium (Lonza) supplemented with 10% FCS, 1% penicillin/streptomycin, 1% L-glutamin, and 1% pyruvate. Cells were used until passage 35 for production of lentiviral particles.
All cell types were cultured at 37 °C in a 5% CO₂ atmosphere and tested negative for mycoplasma.