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Ps32 36 iκbα

Manufactured by Cell Signaling Technology
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PS32/36-IκBα is a primary antibody that specifically recognizes IκBα phosphorylated at Ser32 and Ser36. IκBα is a key regulator of the NF-κB signaling pathway.

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Lab products found in correlation

Pt183 y185 jnk, by Cell Signaling Technology (2 mentions) Pt202 y204 erk, by Cell Signaling Technology (2 mentions) Ps176 180 ikkα β, by Cell Signaling Technology (2 mentions) Py416 c src, by Cell Signaling Technology (2 mentions) Pt180 y182 p38, by Promega (2 mentions) Mouse trip6, by BD (2 mentions) Traf6, by Santa Cruz Biotechnology (2 mentions) C src, by Santa Cruz Biotechnology (2 mentions) Histone h3, by Cell Signaling Technology (2 mentions) Flag tag (flag), by Merck Group (2 mentions) Gapdh, by Santa Cruz Biotechnology (2 mentions)

2 protocols using ps32 36 iκbα

1

Immunoblotting, Co-IP, and Recombinant Protein Purification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Experimental procedures of immunoblotting, co-immunoprecipitation, and purification of recombinant glutathione-S-transferase (GST)-tagged or non-tagged proteins were performed as described previously [19 (link), 34 (link)]. Immunoblotting was performed using antibody specific to human TRIP6, A20 (Bethyl Laboratories), pT183/Y185-JNK, pT202/Y204-ERK, pS32/36-IκBα, pS176/180-IKKα/β, pY416-c-Src, CYLD, IκBα, IKKβ, Histone H3 (Cell Signaling, Danvers, MA, USA), pT180/Y182-p38 (Promega, Madison, WI, USA), ERK, JNK, mouse TRIP6 (BD Biosciences, San Jose, CA, USA), HA (Cayman, Ann Arbor, MI, USA), FLAG (Sigma-Aldrich), GFP, TRAF6, p38, c-Src, GAPDH, GST, ubiquitin, or phosphotyrosine (Santa Cruz Biotechnology, Dallas, TX, USA).
Lin F.T., Lin V.Y., Lin V.T, & Lin W.C. (2016). TRIP6 antagonizes the recruitment of A20 and CYLD to TRAF6 to promote the LPA2 receptor-mediated TRAF6 activation. Cell Discovery, 2, 15048-.
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2

Immunoblotting and Protein Purification Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Experimental procedures of immunoblotting, co-immunoprecipitation, and purification of recombinant glutathione-S-transferase (GST)-tagged or non-tagged proteins were performed as described previously [19 (link), 34 (link)]. Immunoblotting was performed using antibody specific to human TRIP6, A20 (Bethyl Laboratories), pT183/Y185-JNK, pT202/Y204-ERK, pS32/36-IκBα, pS176/180-IKKα/β, pY416-c-Src, CYLD, IκBα, IKKβ, Histone H3 (Cell Signaling, Danvers, MA, USA), pT180/Y182-p38 (Promega, Madison, WI, USA), ERK, JNK, mouse TRIP6 (BD Biosciences, San Jose, CA, USA), HA (Cayman, Ann Arbor, MI, USA), FLAG (Sigma-Aldrich), GFP, TRAF6, p38, c-Src, GAPDH, GST, ubiquitin, or phospho-tyrosine (Santa Cruz Biotechnology, Dallas, TX, USA).
Lin F.T., Lin V.Y., Lin V.T, & Lin W.C. (2016). TRIP6 antagonizes the recruitment of A20 and CYLD to TRAF6 to promote the LPA2 receptor-mediated TRAF6 activation. Cell Discovery, 2, 15048-.
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