Log In Sign up
The largest database of trusted experimental protocols

N6 2 o dibutyryladenosine 3 5 cyclic monophosphate sodium salt dbcamp

Manufactured by Merck Group
Visit Supplier
Sourced in United States, United Kingdom

N6,2'-O-dibutyryladenosine-3',5'-cyclic monophosphate sodium salt (dbcAMP) is a cyclic adenosine monophosphate (cAMP) analog. It functions as a cell-permeable activator of protein kinase A (PKA).

Automatically generated - may contain errors

Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Ab5320, by Merck Group (1 mentions) Mitomycin, by Merck Group (1 mentions) Mca341r, by Bio-Rad (1 mentions) Z0311, by Agilent Technologies (1 mentions) Ab6326, by Abcam (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Ouabain, by Merck Group (1 mentions) Aldosterone, by Merck Group (1 mentions) Digoxin, by Merck Group (1 mentions) Caspase 3, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Digitoxin, by Merck Group (1 mentions) Oleandrin, by Merck Group (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Digitoxigenin, by Merck Group (1 mentions) Dihydroouabain, by Merck Group (1 mentions) Na k atpase α1, by Abcam (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Dulbecco s modified eagle s medium dmem d5796, by Merck Group (1 mentions)

Spelling variants of this product

DbcAMP (146 citations) Dibutyryladenosine 3,5-cyclic monophosphate (2 citations)

4 protocols using n6 2 o dibutyryladenosine 3 5 cyclic monophosphate sodium salt dbcamp

1

Immunochemical Identification of CNS Cell Types

Check if the same lab product or an alternative is used in the 5 most similar protocols
Basic reagents were purchased from Sigma (St Louis, MO), unless indicated otherwise. Bromodeoxyuridine (BrdU; 5-bromo-2′-deoxyuridine) and TIMP-1 from human neutrophil granulocytes were purchased from Calbiochem (San Diego, CA); mitomycin and N6,2′-O-dibutyryladenosine-3′,5′-cyclic monophosphate sodium salt (dbcAMP) were purchased from Sigma; cholera toxin B (CTB) subunit was purchased from List Biological Laboratories (Campbell, CA); and 4′,6-diamidino-2-phenylindole (DAPI) was purchased from Molecular Probes (Eugene, OR). The antibodies used for immunodetection were as follows: monoclonal mouse anti-BrdU (B2531; Sigma) and polyclonal rabbit anti–glial fibrillary acidic protein (GFAP; Z0334; Dako, Carpinteria, CA; for dual labeling with NG2, Iba1, and GFAP); monoclonal rat anti-BrdU (ab6326; Abcam, Cambridge, MA; for dual labeling with O1); polyclonal goat anti-CTB (703; List Biological Laboratories); polyclonal rabbit anti-NG2 (AB5320; EMD Millipore, Billerica, MA); monoclonal mouse anti-O1 (MAB344; Chemicon, Temecula, CA); monoclonal mouse anti-CD11b (MCA618R; Serotec, Raleigh, NC); polyclonal rabbit anti-Iba1 (019-19741; Wako, Richmond, VA); monoclonal mouse rat anti-CD68 (MCA341R; Serotec); polyclonal goat anti–calcitonin gene–related peptide (CGRP; 1720-9007; Biogenesis, Bournemouth, United Kingdom); and polyclonal rabbit anti-S100 (Z0311; Dako).
Liu H., Angert M., Nishihara T., Shubayev I., Dolkas J, & Shubayev V.I. (2015). Spinal Glia Division Contributes to Conditioning Lesion–Induced Axon Regeneration Into the Injured Spinal Cord: Potential Role of Cyclic AMP–Induced Tissue Inhibitor of Metalloproteinase-1. Journal of neuropathology and experimental neurology, 74(6), 500-511.
+ Open protocol
+ Expand
2

Comprehensive Protein Assays and Compound Characterization

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
These assays were performed as described (Yang et al., 2007 (link)) using antibodies against GAPDH, β-actin, and caspase-3 (Cell Signaling Technology Inc., MA, USA); and Na+/K+-ATPase α1 (Abcam Inc. Cambridge, UK). Tylophorine and Reevesiosides A-I were prepared as described (Chang et al., 2013 (link), Yang et al., 2010 (link)). DMSO (D1435, ≧ 99.5%), digoxin (D6003, ≧ 95%, HPLC), digitoxin (D5878, ≧ 92%, HPLC), digitoxigenin (D9404, 99%, TLC), ouabain (O3125, ≧ 95%, HPLC), dihydroouabain (D0670, ≧ 98.5%, TLC), oleandrin (O9640, ≧ 98%, HPLC), N6,2′-O-Dibutyryladenosine 3′,5′-cyclic monophosphate sodium salt (Db cAMP) (D0627, ≧ 96%, HPLC), and aldosterone (A9477, ≧ 95%, HPLC) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Yang C.W., Chang H.Y., Hsu H.Y., Lee Y.Z., Chang H.S., Chen I.S, & Lee S.J. (2017). Identification of anti-viral activity of the cardenolides, Na+/K+-ATPase inhibitors, against porcine transmissible gastroenteritis virus. Toxicology and Applied Pharmacology, 332, 129-137.
+ Open protocol
+ Expand
3

Differentiation of Adipocytes with Hormones

Check if the same lab product or an alternative is used in the 5 most similar protocols
High glucose (25 mM) Dulbecco's modified eagle's medium (DMEM; D5796), cell culture grade water, N6,2′-O-Dibutyryladenosine 3′,5′-cyclic monophosphate sodium salt (dbcAMP), dexamethasone (Dex) and 3,3′,5-Triiodo-l-thyronine sodium salt (T3) were all purchased from Sigma-Aldrich (Poole, UK). Human Recombinant Des(1–3) IGF-I was purchased from SourceBioscience and other cell culture reagents were purchased from Fisher Scientific (Loughborough, UK). Compounds were prepared as follows: dbcAMP was dissolved in cell culture grade water at 100 mM, Des(1–3) IGF-I in 0.1 M acetic acid at 100 μg/ml, T3 in 0.1 M sodium hydroxide at 100 nM and Dex in absolute ethanol at 10 mM. Compounds were filter-sterilised through a 0.2 μm filter and aliquots stored at −20 °C.
Brearley M.C., Loczenski-Brown D.M., Loughna P.T., Parr T, & Brameld J.M. (2021). Response of the porcine MYH4-promoter and MYH4-expressing myotubes to known anabolic and catabolic agents in vitro. Biochemistry and Biophysics Reports, 25, 100924.
+ Open protocol
+ Expand
4

Characterization of EP2 Receptor Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rabbit anti‐EP2 (No. 101750) polyclonal antibody (Ab) was purchased from Cayman Chemical Company. Mouse anti‐GAPDH (CA92590) Ab was from Merck Millipore. Rat anti‐E‐cadherin (ab11512) Ab was from Abcam. The chemical inhibitors PF‐4708671 (PZ0143, 10 μM), indomethacin (I7378, 10–20 μM), L‐798106 (L4545, 10 μM), forskolin (F3917, 10 μM) and N6,2′‐O‐Dibutyryladenosine 3′,5′‐cyclic monophosphate sodium salt (db‐cAMP) (D0627, 1–3 mM) were all purchased from Sigma‐Aldrich; NS398 (ab120295, 10–50 μM) and H‐89 (ab143787, 10–50 μM) were from Abcam; PGE2 (163‐10814, 1–5 μM) was from Wako; SC‐560 (70340, 10–50 μM), ONO‐8711 (14070, 10 μM), PF‐04418948 (15016, 10 μM), butaprost (13740, 10 μM), U‐46619 (16450, 100 nM), 15(s)‐Fluprostenol (16787, 10 μM), sulprostone (14765, 10 μM), BW245C (12050, 10 μM) and Cicaprost (16831, 10 μM) were all from Cayman Chemical Company; ONO‐AE3‐208 (CS‐0315, 10 μM) was from Chemscene; and LY294002 (10 μM) and rapamycin (0.1 μM) were from Calbiochem.
Ishihara E., Nagaoka Y., Okuno T., Kofuji S., Ishigami‐Yuasa M., Kagechika H., Kamimura K., Terai S., Yokomizo T., Sugimoto Y., Fujita Y., Suzuki A, & Nishina H. (2020). Prostaglandin E2 and its receptor EP2 trigger signaling that contributes to YAP‐mediated cell competition. Genes to Cells, 25(3), 197-214.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!