A Spectraphysics Mai Tai Ti:Sapphire tunable laser generates 80 fs excitation pulses centered between 710-820 nm. This excitation pulse propagates toward a pair of chirped mirrors (Femtolasers GSM216), introduced to compensate for any dispersion introduced from other optics. A half wave plate (Thorlabs WPH10M-780) was placed in the beam path and set at the magic angle 54.7° to remove polarization dependence. In order to modulate the power, a neutral density filter was utilized with an OD varying between 0.4 and 0.8. The incident pulse was focused using a 100 mm convex lens (Thorlabs LA1509) into the center of a 1 cm quartz sample cell. The emission of the sample was collected at a right angle by a reflective objective (20 x magnification) and focused into an Ocean Optics USB2000+ UV-VIS Spectrometer. The typical spectral range used on the spectrometer was 180 nm to 870 nm. The excitation wavelengths of 720 nm and 750 nm were chosen for linearity of signal with sample concentration as well as to enable direct comparison of the EuIII complex with the 2PA rhodamine B standard.
Usb2000 uv vis spectrometer
Manufactured by OceanOptics
Sourced in United States
The USB2000 UV-vis spectrometer is a compact, high-performance spectroscopy device designed for a wide range of laboratory applications. It features a ccd detector and a stable, high-intensity light source, providing reliable measurements in the UV-Vis wavelength range.
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7 protocols using usb2000 uv vis spectrometer
1
Time-Resolved Spectroscopy of Eu(III) Complex
2
Customizable LED Optics Enclosure
3
POF-MIP-SPR Sensor for Analyte Detection
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The SPR determinations were made by the D-shaped POF-MIP-SPR sensor. The experimental setup consists of a halogen lamp (HL–2000–LL, Ocean Optics, Dunedin, FL, USA) illuminating the SPR-POF-MIP sensor and a spectrum analyzer (USB2000+UV–VIS spectrometer, Ocean Optics) connected to a computer. The spectral emission of the lamp ranges from 360 nm to 1700 nm and the measurements were performed from 300 nm to 1050 nm. Measurements were performed by dropping a small volume, usually 40 μL, of the appropriate solution on the platform and incubating for 5 min. The light transmission spectrum was obtained. The spectra were normalized to the transmission spectrum obtained at the same platform, before the MIP deposition (bare platform), in the air, since in this dielectric not any plasmon resonance is expected to take place due to the low RI of air [11 (link)]. The transmission minimum corresponds to the resonance wavelength The signal is given by the variation of the resonance wavelength (Δλ, nm) with respect to the resonance wavelength of the blank solution, due to the refractive index variation of the polymeric layer upon the combination of the substrate.
4
Optical Fiber Sensing via Halogen Lamp
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The experimental setup was arranged to measure the transmitted light spectrum and was characterized by a halogen lamp, illuminating the optical sensor systems (POF of 1,000 μm in diameter), and a spectrum analyzer, as shown in Figure 1 .
The employed halogen lamp (Model no. HL-2000-LL, manufactured by Ocean Optics, Dunedin, FL, USA) exhibits a wavelength emission range from 360 nm to 1,700 nm, while the spectrum analyzer detection range was from 200 nm to 850 nm. An Ocean Optics “USB2000+UV-VIS” spectrometer was employed. The spectrometer was finally connected to a computer. The SPR curves along with data values were displayed online on the computer screen and saved with the help of advanced software provided by Ocean Optics.
The employed halogen lamp (Model no. HL-2000-LL, manufactured by Ocean Optics, Dunedin, FL, USA) exhibits a wavelength emission range from 360 nm to 1,700 nm, while the spectrum analyzer detection range was from 200 nm to 850 nm. An Ocean Optics “USB2000+UV-VIS” spectrometer was employed. The spectrometer was finally connected to a computer. The SPR curves along with data values were displayed online on the computer screen and saved with the help of advanced software provided by Ocean Optics.
5
Physicochemical Characterization of Nanomaterials
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Steady-state/transient fluorescence spectrometer (Edinburgh Instruments, FLS-1000, UK), JEM-2100F transmission electron microscope (TEM) (JEOL, Japan), Thermo Scientific K-Alpha+ (XPS) (ThermoFisher), USB-2000 UV–vis spectrometer (Ocean Optics, USA), electronic balance (Shanghai Sunny Hengping Scientific Instrument Co., Ltd., FA2004N), PHS-3W pH meter (Inesa, China), ultrapure water machine (Thermo Fisher, GenPure UV), three-frequency constant-temperature CNC ultrasonic cleaner (KQ-300GVDV, Kunshan Ultrasonic Instrument Factory, Jiangsu Province), magnetic heating plate (IKA Company, RCT basic),
6
Customizable LED Optics Enclosure
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A custom optics housing was 3-D printed as an enclosure (Fig. 2 ). A three-color light-emitting diode (LED) (QT-Brightek 5-mm tri-color round lamp, part number QBL8RGB60D0–2897, qt-brightek.com ) was chosen as the light source for the optical evaluation module. This LED had four electrical connections: a common anode and a separate cathode for each of the three colors. The LED was therefore able to produce red (according to the manufacturer datasheet, peak λ = 624 nm), green (λ = 525 nm), or blue (λ = 470 nm) light with changes only to connections in the electronics, rather than by changing the physical LED component. Only red light was used in this work, while the other two colors were left as options for future work. The emission spectra of the three LED colors were measured on a USB2000 UV-vis spectrometer (Ocean Insight, Orlando, FL, USA) by use of OceanView 2.0.8 software (Ocean Insight).
7
SPR-POF Sensor Performance Comparison
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An SPR fiber sensor has also been used to compare the performance of the new approach with a “classic” plasmonic approach [9 (link)]. Figure 3 shows the SPR-POF sensor with the experimental setup. The plasmonic platform was realized as described in [9 (link),26 (link)]. In this SPR sensor, the length of the sensing region is about 10 mm. The photoresist layer is 1500 nm thick and the gold film is 60 nm, presenting a good adhesion to the substrate too. In this configuration (SPR-POF sensor), we have used a more expensive “spectral interrogation” [9 (link),26 (link)]. Here, the experimental setup consists of a halogen lamp (HL-2000-LL, Ocean Optics, Dunedin, FL, USA) illuminating the sensor and a spectrum analyzer (USB2000+UV–VIS spectrometer, Ocean Optics, Dunedin, FL, USA) connected to a computer, as shown in Figure 3 . The spectral emission of the lamp ranges from 360 nm to 1700 nm and the spectrometer is sensitive from 300 nm to 1050 nm.
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