2 × 104 cells (SNU1 and KATO III) were seeded in 96-well plates as described above. Then, the cells were supplemented with QS-21 (5 µM) or QA (100 µM), and incubated during 24 h. Activities of caspases 3 and 7 upon incubation of the cells were detected using the Caspase-Glo 3/7 assay following the manufacturer’s instructions. The fluorescence of the lysates (proportional to the amount of caspases activity present) was measured at 510 nm in an Appliskan multi-well fluorescence plate reader (Thermo Fischer Scientific, Waltham, MA, USA).
Appliskan multi well fluorescence plate reader
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Appliskan multi-well fluorescence plate reader is a laboratory instrument designed to measure fluorescence intensity in multi-well microplates. It is capable of detecting and quantifying fluorescent signals from a variety of samples, such as cell-based assays, protein detection, and nucleic acid analysis.
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Lab products found in correlation
2 protocols using appliskan multi well fluorescence plate reader
1
Quantifying Caspase Activity in Cancer Cells
2
Quantifying Oxidative Stress in Rat Spermatogenic Cells
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Rat spermatogenic cells were resuspended in KH-Ca2+-lactate media to a final concentration of 6 x 105 cells/mL. Cells were pre-incubated for 3 min at 33°C and then H2DCFDA (2.5 μM final concentration) was added to the cell suspension. Fluorescence measurements with an excitation at 495 nm and emission at 529 nm were made of dichlorofluorescein (DCF) (which is the product of H2DCFDA oxidation) at 15 s intervals for 15 min in an Appliskan multiwell fluorescence plate reader (Thermo Fisher Scientific, Inc.). Standardization of the probe and data analysis was performed as described by [39 (link)].
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