Lysis buffer

Manufactured by Abbott

Sourced in United States

Lysis buffer is a solution used to break down and disrupt the cell membrane and other cellular components, facilitating the extraction and isolation of biological molecules such as proteins, nucleic acids, and other cellular contents. The core function of lysis buffer is to enable the release of these intracellular components for subsequent analysis or purification.

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Lab products found in correlation

Realtime hiv 1 assay, by Abbott (1 mentions)

2 protocols using lysis buffer

Tissue Homogenization for CMV Quantification

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The sample weight was measured under sterile conditions. The samples were then mixed with 1000 μL of lysis buffer (Abbott, Chicago, IL, USA) and gently shaken in a water bath at 45 °C for 6 h to homogenize them [13 (link)]. The following steps were carried out in a similar manner to those for the plasma samples. Finally, the CMV load per milligram of tissue was determined based on the qPCR results.

Esen S., Saglik I., Dolar E., Cesur S., Ugras N., Agca H., Merdan O, & Ener B. (2024). Diagnostic Utility of Cytomegalovirus (CMV) DNA Quantitation in Ulcerative Colitis. Viruses, 16(5), 691.

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HIV-1 RNA Quantitation from Plasma

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Plasma and RT HIV-1 RNA quantitation was performed at the Emory Center for AIDS Research Virology and Molecular Biomarkers Core Laboratory using the Abbott Real-Time HIV-1 Assay with detectable range of 40-10,000,000 copies/mL. The RT HIV RNA assay was developed and optimized with methodologies used previously in other biological matrices[11 ]. RT was weighed, combined with 500 mL of Promega lysis buffer and 25 uL Abbott Proteinase K, and incubated at 56°C until dissolved. The supernatant was removed, volume brought up to 1 mL with phosphate-buffered saline, and run on the Abbott Realtime HIV-1 assay with results reported as RNA copies per gram.

LAHIRI C.D., BROWN N.L., RYAN K.J., ACOSTA E.P., SHETH A.N., MEHTA C.C., INGERSOLL J, & OFOTOKUN I. (2018). HIV RNA persists in rectal tissue despite rapid plasma virologic suppression with dolutegravir-based therapy. AIDS (London, England), 32(15), 2151-2159.

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