Mouse albumin enzyme linked immunosorbent assay kit

Manufactured by Fortis Life Sciences

Sourced in United States

The Mouse Albumin Enzyme-Linked Immunosorbent Assay (ELISA) Kit is a quantitative in vitro assay designed to measure the concentration of albumin in mouse biological samples.

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Mcp 1, by Thermo Fisher Scientific (1 mentions)

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Mouse albumin

3 protocols using mouse albumin enzyme linked immunosorbent assay kit

Quantifying Urinary Albumin and Creatinine

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Albumin and creatinine levels in the urine of mice were measured as previously described [19 (link),20 (link)]. Mice were placed in individual metabolic cages for 12 h to collect urine samples after 24 weeks of STZ-induced hyperglycaemia. Albuminuria was determined using a mouse albumin enzyme-linked immunosorbent assay kit (Bethyl Laboratories) according to the manufacturer’s instructions. Urinary creatinine was determined using the Cobasc501 module assay from Roche Diagnostics [14 (link)].

Rana R., Manoharan J., Gupta A., Gupta D., Elwakiel A., Khawaja H., Fatima S., Zimmermann S., Singh K., Ambreen S., Gadi I., Biemann R., Jiang S., Shahzad K., Kohli S, & Isermann B. (2022). Activated Protein C Ameliorates Tubular Mitochondrial Reactive Oxygen Species and Inflammation in Diabetic Kidney Disease. Nutrients, 14(15), 3138.

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Measuring Urinary Albumin in Mice

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The level of urine albumin was measured using the mouse albumin enzyme-linked immunosorbent assay kit (catalog no. E99-134, Bethyl Laboratories Inc., Montgomery, TX, United States), according to the manufacturer’s instructions. Twenty-four-hour mouse urine was collected with mouse metabolic cage while restricting food.

Wu C., Yan F., Li M., Tu Y., Guo Z., Chen Y., Wu Y., Li Q., Yu C., Fu Y., Wu M., Kong W., Gao Y., Li X, & Zheng J. (2021). Whole-Mount Kidney Clearing and Visualization Reveal Renal Sympathetic Hyperinnervation in Heart Failure Mice. Frontiers in Physiology, 12, 696286.

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Quantifying Retinal Vascular Leakage

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Retinal vascular leakage was measured by quantitating albumin levels in single retina (excluding RPE/choroid) according to previously published method 23 by using a mouse albumin enzyme-linked immunosorbent assay kit (Bethyl Laboratories, Montgomery, TX). Values were normalized to dry retinal weight, and four to six mice per group were evaluated. With the use of an established protocol in cultured rat Müller cells, 33 protein levels of monocyte chemoattractant protein-1 (Mcp1; eBioscience, San Diego, CA) were evaluated in cell supernatant.

Alrashdi S.F., Deliyanti D., Talia D.M, & Wilkinson-Berka J.L. (2018). Endothelin-2 Injures the Blood-Retinal Barrier and Macroglial Müller Cells: Interactions with Angiotensin II, Aldosterone, and NADPH Oxidase. The American journal of pathology, 188(3).

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