Cells were labeled with antibody or the corresponding isotype control in 1%BSA/PBS for 30 mins on ice. Cells were washed and analyzed using an Accuri C6 flow cytometer (BD Bioscience); histograms were generated using FlowJo software. Mean fluorescence values were normalized to the control cell line level. Antibodies used were CD82-Alexa-647 (Biolegend, ASL-24), CD53-PE (BioLegend, HI29), and Integrin β1-Alexa-647 (BioLegend, TS2/16). For active β1 integrin expression, cells were labeled with Ligand-induced binding site (LIBS) Anti-Integrin β1 Antibody (Millipore, HUTS-4) for 30 mins. Cells were washed and labeled with goat anti-mouse Alexa-647 secondary antibody (Invitrogen).
Asl 24
Manufactured by BioLegend
The ASL-24 is a laboratory instrument designed for automated cell separation and labeling. It is capable of processing multiple samples simultaneously, allowing efficient and consistent sample preparation for downstream applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using asl 24
1
Flow Cytometric Analysis of Cell Surface Markers
2
Flow Cytometric Analysis of Cell Surface Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were labeled with antibody or the corresponding isotype control in 1%BSA/PBS for 30 mins on ice. Cells were washed and analyzed using an Accuri C6 flow cytometer (BD Bioscience); histograms were generated using FlowJo software. Mean fluorescence values were normalized to the control cell line level. Antibodies used were CD82-Alexa-647 (Biolegend, ASL-24), CD53-PE (BioLegend, HI29), and Integrin β1-Alexa-647 (BioLegend, TS2/16). For active β1 integrin expression, cells were labeled with Ligand-induced binding site (LIBS) Anti-Integrin β1 Antibody (Millipore, HUTS-4) for 30 mins. Cells were washed and labeled with goat anti-mouse Alexa-647 secondary antibody (Invitrogen).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!