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4 protocols using soybean lecithin

1

NaCh Hydrate-based Lipid Formulation

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NaCh hydrate was obtained from Beijing Budweiser Technology CO., Ltd. Soybean lecithin was obtained from Tokyo Chemical Industry CO., Ltd. The molecular structures of NaCh and Soybean lecithin are shown in Scheme 1. Medium-chain triacylglycerols (MCT) were obtained from Shanghai Yuanye Bio-Technology Co., Ltd. Tris (hydroxymethyl) methyl aminomethane was obtained from Shanghai Macklin Bio-Technology Co., Ltd. Hydrochloric acid (HCl) and NaOH were obtained from Sinopharm Chemical Reagent Co., Ltd. Sodium chloride (NaCl) and calcium chloride (CaCl 2 ) were obtained from Beijing J&K Scientific Ltd. Lipase was obtained from Shanghai Yuanye Bio-Technology Co., Ltd. Ultrapure water with a resistivity of 18.2 MΩ.cm was used for the preparations of solutions. n-Decane, with a purity of 99.5%, was obtained from Beijing Budweiser Technology CO., Ltd. All the other chemicals used in this study were of analytical grade.
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2

Coating Solutions for Fruit Preservation

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Figs cv. ‘Pingo de mel’ were harvest (handpicked) early in the morning, at commercial ripening stage, in September 2018, from an orchard of Santarém district, Portugal (39.507381, −8.642617). Fruits were maintained under ambient conditions (22–25 °C) for about 8 h before the essays, due to the logistic conditions available.
For coating solutions preparation, chitosan and sodium alginate were purchased from Golden/Shell Biochemical Co. Ltd. (Zhejiang, China) and Quimidroga, S.A. (Barcelona, Spain), respectively. Glycerol was acquired from Fisher Scientific (Loughborough, UK), lactic acid from Panreac Quimica SAU (Barcelona, Spain), and calcium chloride from Absolve® (Portugal). The soybean lecithin was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan), and olive oil (Lisbon, Portugal) was acquired at a local store.
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3

Formulation and Characterization of Amphotericin B Lipid Nanocarriers

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Amphotericin B was procured from Enke Pharma® (Cangzhou Enke Pharma-tech co, Ltd, Hebei, China). Olive oil and poly(vinyl alcohol) (PVA) of molecular weight 9000–10,000 Da were purchased from Sigma-Aldrich (Chemie GmbH, Japan). Soyabean oil and oleic acid were purchased from Sigma-Aldrich (Chemie GmbH, USA). Sesame oil was purchased from Sigma-Aldrich (Chemie GmbH, Mexico). Castor oil was purchased from Ransom Natural Ltd (Hitchin, England). Campul PG-8/NF® (propylene glycol monocaprylate) and Campul MCM C-8 EP/NF (glyceryl monocaprylate) were kindly gifted by ABITEC Corporation (Wisconsin, USA). Polyoxyethylene sorbitan monooleate (Tween®80) was obtained from VWR Chemical® (Solon, Ohia, USA). Soybean Lecithin was supplied from Tokyo Chemical Industry co, Ltd (Tokyo, Japan). Poly(vinylpyrrolidone) (PVP) of molecular weight 580,000 Da (K-29/32) was purchased from Ashland Industries (Wilmington, USA). Purified water utilized in all experiments was obtained from ELGA® DV 25, Purelab Option, water purification System (ELGA-Q, USA). All the other chemicals and reagents were of analytical grade.
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4

Physiochemical Characterization of Biomaterials

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The following chemicals were of either an analytical or reagent grade and used without further pretreatment. IR-820 dye (CAS No 172616–80-7) denoted as “IR”, tetramethylrhodamine isothiocyanate-dextran (TRITC-Dex40; MW = 40kDa), phenol (CAS No 108–95-2), 5-bromo-4-chloro-3-indolyl phosphate disodium salt (BCIP; CAS No 38404–93-2), and Triton® X-100 (CAS No 9002–93-1) were purchased from Sigma-Aldrich Co (Missouri, USA). Soybean lecithin (CAS No 8002–43-5), cholesterol (CAS No 57–88-5), sodium deoxycholate (CAS No 302–95-4), 9,10-dimethylanthracene (DMA; CAS No 781–43-1), and gallic acid hydrate (CAS No 5995–86-8) were obtained from Tokyo Chemical Industry Co, Ltd (Tokyo, Japan). Ammonium ferrous sulfate hexahydrate (CAS No 7783–85-9), butylated hydroxytoluene (BHT; CAS No 128–37-0), and xylenol orange (CAS No 3618–43-7) were obtained from Loba Chemie Pvt Ltd (Mumbai, India). Acetone (CAS No 67–64-1), chloroform (CAS No 67–66-3), and methanol (CAS No 67–56-1) were supplied from VWR International Company (Pennsylvania, USA), RCI Labscan Limited. (Bangkok, Thailand), and Fisher Scientific Co LLC (Pennsylvania, USA), respectively. Type 1 (ultrapure) water was produced by using an arium® pro ultrapure water system (Sartorius AG, Germany) to maintain the electrical conductivity at 0.055 μS/cm at room temperature and was used for all the experiments.
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