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6 protocols using sodium acetate

1

Antioxidant and Cytotoxicity Assessment

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The following substances were used: 2,2-diphenyl-1-picrylhydrazyl, 5,5′-dithiobis (2-nitrobenzoic acid), bovine serum albumin, glutathione, butylated hydroxytoluene, Griess reagent, MTT, pyrogallol, xylenol orange (all from Sigma, St. Louis, USA), absolute ethanol, acetic acid, ascorbic acid, ferrous ammonium sulfate, hydrochloric acid, formaldehyde, hydrogen peroxide, methanol, sodium acetate, trichloroacetic acid (Vetec, Rio de Janeiro, RJ, Brazil), sulfuric acid, dimethyl sulfoxide and N,N-dimethylformamide (DMSO, Synth, Diadema, SP, Brazil), Dulbecco's Modified Eagle Medium (DMEM, Vitrocell, Campinas, SP, Brazil), fetal bovine serum (FBS, Gibco), and dextran sulfate sodium (Alfa Aesar, Heysham, Lancashire, UK).
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2

Gellan Gum-based Topical Formulations

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Gellan gum low acyl was kindly donated by CP Kelco (Atlanta, GA, USA). Ethylcellulose 10 (Ethocel™ Standard 10 Premium) was donated by Colorcon® (Cotia, Brazil). Oleic acid (OA) (about 78% purity) was purchased from LabSynth (Diadema, Brazil). Sorbitan monooleate 80 (Span® 80), polysorbate 80 (Tween® 80), hexadecyltrimethylammonium bromide (HTAB), and tetramethylbenzidine (TMB) were obtained from Sigma-Aldrich (São Paulo, Brazil). Dexamethasone acetate was purchased from Nova Derme pharmacy (Santa Maria, Brazil). Ketamine (Dopalen®) and xylazine (Anasedan®) were obtained from Ceva (Paulínia, Brazil). Sodium citrate, sodium acetate, and acetone were purchased from Vetec (Rio de Janeiro, Brazil). Laboratory kits for biochemical tests were purchased from Labtest Diagnóstica (Lagoa Santa, Brazil), Katal Biotecnologia (Belo Horizonte, Brazil), and Wiener Lab (São Paulo, Brazil). Methanol high-performance liquid chromatography (HPLC) grade was obtained from Merck (Barueri, Brazil). acetone analytical grade was purchased from Dinâmica Química Contemporânea (Indaiatuba, Brazil). All other reagents and solvents were of analytical grade and used as received.
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3

Assessing Anti-Inflammatory Properties of Natural Compounds

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The following drugs were used to execute the experimental protocols: Croton oil, hexadecyltrimethylammonium bromide (HTAB), tetramethylbenzidine (TMB), dexamethasone, rutin, gallic acid, ascorbic acid, chlorogenic acid, rosmarinic acid, solanine, DPPH, ammonium phosphate monobasic (all from Sigma, St. Louis, MO, USA), hematoxylin-eosin, acetonitrile, butanol, paraffin, ethanol, and Folin-Ciocalteu (all from Merck, Whitehouse Station, New Jersey, USA), Isoflurane (Baxter, São Paulo, Brazil), sodium acetate, acetone, acetic acid, and formaldehyde (all from Vetec, Rio de Janeiro, Brazil).
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4

Synthesis and Characterization of Metal-Organic Compounds

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Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl), PEI (branched, MW 800 Da), chloramine-T hydrate, and phenol red sodium salt were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dopamine hydrochloride (DA-HCl) was purchased from Acros Organic (Geel, Belgium). Potassium hydroxide, hydrochloride, sodium thiosulfate, and potassium bromide were purchased from SHOWA (Gunma, Japan). Ethylenediamine (En), anhydrous cobalt chloride, silver nitrite, and anhydrous copper chloride were purchased from Alfa Aesar (Tewksbury, MA, USA). Sodium chloride was purchased from Fluka (Munich, Germany). Sodium acetate was purchased from Vetec (St. Louis, MO, USA). All these chemicals were of analytical grade and were used without further purification. The PPMM (catalog number: 201PP-47-045-50; diameter of 47 mm; pore size of 450 nm; thickness of 200 µm) was purchased from Rone Scientific Inc. (New Taipei City, Taiwan).
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5

Antioxidant Compounds Characterization

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2,4-Dinitrophenylhydrazine (DNPH), 1,1-Diphenyl-2-picrylhydrazyl (DPPH), butylated hydroxyltoluene (BHT), 2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid (ABTS), 6-hydroxy-2,5,7,8-tetramethylchroman-2carboxylic acid (Trolox), quercetin, catechin, and β-carotene were obtained from Sigma Chemical Co. (MO, USA). Potassium persulfate, tween 40, Folin-Ciocalteau, sodium carbonate from Dinamina, sulfuric acid, methanol, ethanol, hydrochloric acid, ascorbic acid, chloroform, linolenic acid, gallic acid, aluminum chloride, sodium acetate, and vanillin were obtained from Vetec (RJ, Brazil).
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6

Antioxidant and Anti-Inflammatory Assays

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Dexamethasone (DEXA), 12-O-tetradecanoylphorbol-acetate (TPA), hexadecyl-trimethylammonium bromide (HTAB), tetramethylbenzidine (TMB) all from Sigma Chemcil Co (St Louis MO); acetone, propanone, formaldehyde, glacial acetic acid, paraffin, dimethylformamide, phosphate-buffered (PBS) 2′,7′-dichlorodihydrofluorescein diacetate (DCHF-DA), 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), ascorbic acid were purchased from Merck Biosciences (Germany); hydrogen peroxide, sodium acetate, ethanol, hematoxylin, and eosin were purchased from Vetec (Rio de Janeiro, Brazil). The other chemical reagents of analytical grade were purchased from local chemical suppliers.
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