Nis element d 3.2 image analysis software

Manufactured by Nikon

Sourced in Japan

NIS-Element-D 3.2 is an image analysis software developed by Nikon. It provides tools for acquiring, processing, and analyzing digital images from various microscopy techniques. The software offers a range of image analysis functions, including measurement, annotation, and quantification of various parameters.

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Lab products found in correlation

Vimentin, by Agilent Technologies (1 mentions)

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NIS-Elements software (2071 citations) NIS-Elements (1183 citations) NIS-Element software (210 citations) NIS-Elements D 3.2 (68 citations) NIS-Elements 3.2 (60 citations) NIS-Elements D software (52 citations) NIS-Elements D (45 citations) NIS-Elements analysis software (36 citations) NIS-Elements image analysis software (22 citations) Elements analysis software (14 citations)

2 protocols using nis element d 3.2 image analysis software

Quantifying Thrombus Composition Using Image Analysis

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The sizes of the thrombi were measured in sections under a microscope using the NIS-Element-D 3.2 image analysis software (Nikon, Tokyo, Japan). Areas that were immunopositive for CD34, fibrin, integrin α2bβ3, glycophorin A, and SMA were semiquantified using the Win Roof color image analysis software (Mitani, Fukui, Japan) (Fig. 2) [10 (link)]. These areas are expressed as the ratios of positively stained areas per thrombus area. The number of granulocytes without lytic change was counted in the five most cellular fields under a 20× objective lens, and is expressed as the number per mm². The immunopositive cell numbers for CD68, CD163, and CD206 in venous thrombi were counted in the five most heavily stained fields under a 20× objective lens. Cell density is expressed as the number of immunopositive cells per mm².Fig. 2

Representative immunohistochemical image of glycophorin A and its color-extracted image in image analysis. Immunopositive areas were extracted using specific protocols based on the color parameters of hue, lightness, and saturation. The data are expressed as ratios (%) of the extracted light green areas in the areas of thrombus

Furukoji E., Gi T., Yamashita A., Moriguchi-Goto S., Kojima M., Sugita C., Sakae T., Sato Y., Hirai T, & Asada Y. (2016). CD163 macrophage and erythrocyte contents in aspirated deep vein thrombus are associated with the time after onset: a pilot study. Thrombosis Journal, 14, 46.

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Comparative Spinal Cord Pathology in HAM/TSP and ATL

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Spinal cord autopsy specimens were compared between HAM/TSP (Case 1) and ATL (Case 2) to verify that pathological changes were due to HAM/TSP rather than HTLV-1 infection. The clinical courses of these patients are described below. Specimens were obtained perpendicular to the spinal cord at the C2, C4, C6, and T2 vertebral levels. Staining using hematoxylin and eosin (HE), Klüver-Barrera, and AZAN were performed in the qualitative assessment. Furthermore, in HE-stained slices, APD, TD, RAT, and the area of total (=CSA), gray matter, white matter, anterior column, posterior column, and lateral column were measured for quantitative evaluation. The ratios of these areas to the total area were also calculated. Additionally, the degree of gliosis was evaluated with immunohistochemical staining for vimentin (DAKO, Glostrup, Denmark). The individual areas of each specimen were measured using NIS-Element-D 3.2 image analysis software (Nikon, Tokyo, Japan). The number of vimentin-immunopositive stellate cells was counted under a microscope (BX51, Olympus, Tokyo, Japan).

Taniguchi A., Mochizuki H., Yamashita A., Shiomi K., Asada Y, & Nakazato M. (2017). Spinal cord anteroposterior atrophy in HAM/TSP: Magnetic resonance imaging and neuropathological analyses. Journal of the neurological sciences, 381.

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