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Buv496

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BUV496 is a fluorochrome-conjugated antibody produced by BioLegend for use in flow cytometry applications. It has an excitation maximum of 496 nm and an emission maximum of 543 nm.

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Lab products found in correlation

Apc cy7, by BioLegend (4 mentions) Bv780, by BioLegend (4 mentions) Bb700, by BD (4 mentions) Bv610, by BioLegend (4 mentions) Buv563, by BD (4 mentions) Buv805, by BD (4 mentions) Collagenase type 4, by Worthington (4 mentions) Facsymphony analyzer, by BD (3 mentions) Facsdiva, by BD (2 mentions)

Close spelling variants of this product

CD8 BUV496 Anti–CD4-BUV496 CD3 BUV496

4 protocols using buv496

1

Profiling Immune Cell Populations in Inguinal Lymph Nodes

Cited 2 times
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Draining inguinal lymph nodes from immunized mice were collected and treated as previously described (47 (link), 48 (link)). Briefly, the tissues were treated with collagenase type IV (Worthington) at a concentration of 1 mg/mL for 20 min at a temperature of 37 °C, and then passed through a 100-μm strainer to obtain a single-cell suspension. The resulting single-cell samples were stained with a range of markers including Zombie UV (BUV496, BioLegend 423107), anti-Ly6C (BV780, BioLegend 128041), anti-Ly6G (APC-Cy7, BioLegend 127624), anti-CD19 (BUV395, BD 563557), anti-CD3 (BB700, BD742175), anti-MHCII (AF700, eBioscience 56-5321-82), anti-CD11b (BV650, BioLegend 101239), anti-CD11c (BV421, BioLegend 117330), anti-CD86 (A647, BioLegend 105020), anti-Siglec-F (PE-CF594, BD 562757), anti-CD45 (BV610, BioLegend 103140), anti-CD169 (PE-Cy7, BioLegend 142412), anti-PDCA-1 (BUV563, BD 749275), anti-CD8a (BUV805, BD 612898), anti-CD103 (PE, eBioscience 12-1031-82), anti-NK1.1 (BV510, BioLegend 108738), and anti-F4/80 (BUV737, BD 749283). The cells were analyzed using the BD FACSymphony analyzer located at the Stanford Shared Fluorescence-activated cell sorting (FACS) Facility.
Amaya L., Grigoryan L., Li Z., Lee A., Wender P.A., Pulendran B, & Chang H.Y. (2023). Circular RNA vaccine induces potent T cell responses. Proceedings of the National Academy of Sciences of the United States of America, 120(20), e2302191120.
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2

Comprehensive Immune Cell Profiling of Vaccinated Mice

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Draining iliac lymph nodes from BNT162b2 immunized mice, draining inguinal lymph nodes from YF-17D immunized mice, along with whole lung or spleen, were harvested and digested with 1 mg/mL collagenase type IV (Worthington) for 20 min at 37 °C, followed by smashing with a 100 μm strainer to make a single-cell suspension. Red blood cells from the lung and spleen were lysed before staining. Single-cell samples were then stained with Zombie UV™ (1:300, BUV496; BioLegend #423107), anti-Ly6C (1:500, BV780; BioLegend #128041), anti-Ly6G (1:400, APC-Cy7; BioLegend #127624), anti-CD19 (1:100, BB700; BD #566411), anti-CD3 (1:100, BB700; BD #742175), anti-MHCII (1:400, AF700; eBioscience #56-5321-82), anti-CD11b (1:300, BV650; BioLegend #101239), anti-CD11c (1:400, BV421; BioLegend #117330), anti-CD86 (1:300, A647; BioLegend #105020), anti-Siglec-F (1:400, PE-CF594; BD #562757), anti-CD45 (1:200, BV610; BioLegend #103140), anti-CD169 (1:200, PE-Cy7; BioLegend #142412), anti-PDCA-1 (1:200, BUV563; BD #749275), anti-CD8a (1:200, BUV805; BD #612898), anti-CD103 (1:100, PE; eBioscience #12-1031-82), anti-NK1.1 (1:200, BV510; BioLegend #108738), and anti-F4/80 (1:100, BUV737; BD #749283). Data was collected on a BD FACSymphony analyzer with BD FACSDiva (v8.0.1).
Li C., Lee A., Grigoryan L., Arunachalam P.S., Scott M.K., Trisal M., Wimmers F., Sanyal M., Weidenbacher P.A., Feng Y., Adamska J.Z., Valore E., Wang Y., Verma R., Reis N., Dunham D., O’Hara R., Park H., Luo W., Gitlin A.D., Kim P., Khatri P., Nadeau K.C, & Pulendran B. (2022). Mechanisms of innate and adaptive immunity to the Pfizer-BioNTech BNT162b2 vaccine. Nature immunology, 23(4), 543-555.
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3

Comprehensive Immune Profiling of Iliac Lymph Nodes

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Iliac lymph nodes were harvested at day 1 post-vaccination. LNs were treated with 5 mg/ml collagenase type IV (Worthington) for 20 min at 37 °C and smashed against a 100μm strainer to make a single-cell suspension. Cells were then stained with Zombie UV™ (BUV496; Biolegend # 423108, 1:250 dilution), anti-CD64 (A488; Biolegend # 139316, 1:100 dilution), anti-Ly6C (BV780; Biolegend # 128041, 1:500 dilution), anti-Ly6G (APC-Cy7; Biolegend# 127624, 1:400 dilution), anti-CD19 (BB700; BD # 566411, 1:200 dilution), anti-CD3 (BB700; BD #742175, 1:200 dilution), anti-MHCII (AF700; eBioscience #56-5321-82, 1:400 dilution), anti-CD11b (BV650; Biolegend #101239, 1:300 dilution), anti-CD11c (BV421; Biolegend #117330, 1:400 dilution), anti-CD86 (A647; Biolegend #105020, 1:300 dilution), anti-Siglec-F (PE-CF594; BD #562757, 1:400 dilution), anti-CD24 (BUV395; BD #744471, 1:200 dilution), anti-CD45 (BV610; Biolegend #103140, 1:300 dilution), anti-CD169 (PE-Cy7; Biolegend #142412, 1:200 dilution), anti-PDCA-1 (BUV563; BD #749275, 1:200 dilution), anti-CD8a (BUV805; BD #612898, 1:200 dilution), anti-CD103 (PE; eBioscience #12-1031-82, 1:100 dilution), anti-NK1.1 (BV510; Biolegend #108738, 1:200 dilution), anti-F4/80 (BUV737; BD #749283, 1:100 dilution). Cells were analyzed with the BD FACSymphony analyzer at Stanford HIMC core.
Grigoryan L., Lee A., Walls A.C., Lai L., Franco B., Arunachalam P.S., Feng Y., Luo W., Vanderheiden A., Floyd K., Wrenn S., Pettie D., Miranda M.C., Kepl E., Ravichandran R., Sydeman C., Brunette N., Murphy M., Fiala B., Carter L., Coffman R.L., Novack D., Kleanthous H., O’Hagan D.T., van der Most R., McLellan J.S., Suthar M., Veesler D., King N.P, & Pulendran B. (2022). Adjuvanting a subunit SARS-CoV-2 vaccine with clinically relevant adjuvants induces durable protection in mice. NPJ Vaccines, 7, 55.
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4

Comprehensive Immune Cell Profiling of Vaccinated Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Draining iliac lymph nodes from BNT162b2 immunized mice, draining inguinal lymph nodes from YF-17D immunized mice, along with whole lung or spleen, were harvested and digested with 1 mg/mL collagenase type IV (Worthington) for 20 min at 37 °C, followed by smashing with a 100 μm strainer to make a single-cell suspension. Red blood cells from the lung and spleen were lysed before staining. Single-cell samples were then stained with Zombie UV™ (1:300, BUV496; BioLegend #423107), anti-Ly6C (1:500, BV780; BioLegend #128041), anti-Ly6G (1:400, APC-Cy7; BioLegend #127624), anti-CD19 (1:100, BB700; BD #566411), anti-CD3 (1:100, BB700; BD #742175), anti-MHCII (1:400, AF700; eBioscience #56-5321-82), anti-CD11b (1:300, BV650; BioLegend #101239), anti-CD11c (1:400, BV421; BioLegend #117330), anti-CD86 (1:300, A647; BioLegend #105020), anti-Siglec-F (1:400, PE-CF594; BD #562757), anti-CD45 (1:200, BV610; BioLegend #103140), anti-CD169 (1:200, PE-Cy7; BioLegend #142412), anti-PDCA-1 (1:200, BUV563; BD #749275), anti-CD8a (1:200, BUV805; BD #612898), anti-CD103 (1:100, PE; eBioscience #12-1031-82), anti-NK1.1 (1:200, BV510; BioLegend #108738), and anti-F4/80 (1:100, BUV737; BD #749283). Data was collected on a BD FACSymphony analyzer with BD FACSDiva (v8.0.1).
Li C., Lee A., Grigoryan L., Arunachalam P.S., Scott M.K., Trisal M., Wimmers F., Sanyal M., Weidenbacher P.A., Feng Y., Adamska J.Z., Valore E., Wang Y., Verma R., Reis N., Dunham D., O’Hara R., Park H., Luo W., Gitlin A.D., Kim P., Khatri P., Nadeau K.C, & Pulendran B. (2022). Mechanisms of innate and adaptive immunity to the Pfizer-BioNTech BNT162b2 vaccine. Nature immunology, 23(4), 543-555.
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