Total protein concentrations of lung homogenate lysates were analyzed with a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). SDS-PAGE was performed using Mini-PROTEAN® Precast Mini PAGE Gels (Bio-Rad, Hercules, CA). Trans-Blot Turbo Mini 0.2 µM PVDF Transfer Packs (Bio-Rad) were used for transferring of proteins to the PVDF membranes. Membranes were blocked for 3 h at RT and incubated with primary antibodies (rabbit anti-mouse NF-κB(RelA/p65/) (sc-8008; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-mouse phospho (p)-RelA/p65(NF-κB) (Ser276; A1953; Abcam), mouse anti-mouse arginase-1 (ab239731), rabbit anti-mouse CD206 (ab64693) and rabbit anti-mouse GAPDH (14C10; Cell Signaling; 1:500) in blocking buffer. After washing with PBS-Tween, membranes were incubated with secondary antibodies (Alexa Fluor 488-conjugated goat anti rabbit/mouse (Invitrogen, Carlsbad, CA)) for 1 h RT. Imaging of blots was preformed using a ChemiDoc system (Bio-Rad) followed by quantification with densitometry normalized to GAPDH.
Alexa fluor 488 conjugated goat anti rabbit mouse
Manufactured by Thermo Fisher Scientific
Alexa Fluor 488-conjugated goat anti rabbit/mouse is a secondary antibody conjugated with the Alexa Fluor 488 fluorescent dye. It is designed for use in immunofluorescence applications to detect and localize rabbit or mouse primary antibodies.
Automatically generated - may contain errors
Lab products found in correlation
Mini protean precast mini page gels, by Bio-Rad (2 mentions)
Chemidoc system, by Bio-Rad (2 mentions)
Trans blot turbo mini 0.2 m pvdf transfer packs, by Bio-Rad (2 mentions)
Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Rabbit anti mouse gapdh, by Cell Signaling Technology (1 mentions)
Rabbit anti mouse gapdh, by Thermo Fisher Scientific (1 mentions)
2 protocols using alexa fluor 488 conjugated goat anti rabbit mouse
1
Western Blot Analysis of Lung Proteins
2
Quantifying Lung Protein Levels via Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total protein concentrations of lung homogenate lysates were determined and SDS-PAGE was performed using Mini-PROTEAN® Precast Mini PAGE Gels (Bio-Rad, Hercules, CA). Trans-Blot Turbo Mini 0.2 µM PVDF Transfer Packs (Bio-Rad) were used for transferring of proteins to the PVDF membranes. Membranes were blocked for 3h at RT and incubated with primary antibodies (rabbit anti-human TRAP5b (provided by Dr. Göran Andersson), rabbit polyclonal to TRAP5 (Cat #PA5-116970; Invitrogen, MA, USA), and rabbit anti-mouse GAPDH (Cat #MA5-15738-D680; Invitrogen 1:500) in blocking buffer. After washing with PBS-Tween, membranes were incubated with secondary antibodies (Alexa Fluor 488-conjugated goat anti rabbit/mouse (Invitrogen, Carlsbad, CA) for 1h RT. Imaging of blots was preformed using a ChemiDoc system (Bio-Rad) followed by quantification with densitometry normalized to GAPDH.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!