The data were analyzed using Microsoft Office Excel 2013 and GraphPad Prism 6.01 (GraphPad Software Inc, San Diego, CA) software. Data were presented as mean ± standard deviation (SD) of 3 replicates.
Microsoft office excel 2013
Manufactured by GraphPad
Sourced in United States
Microsoft Office Excel 2013 is a spreadsheet software application that allows users to organize, analyze, and visualize data. It provides tools for creating, formatting, and manipulating tabular data, performing calculations, and generating charts and graphs.
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Lab products found in correlation
5 protocols using microsoft office excel 2013
1
Excel and GraphPad Analysis of Triplicates
2
Comparative Statistical Analysis of Experimental Data
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Analyses were performed using Microsoft Office Excel 2013, Graph Pad 9.3.0 (GraphPad Software Inc., La Jolla, CA, USA) and R (4.2.3). Differences between and in groups were analyzed using the Friedman test (paired, non-normal distribution), Wilcoxon test (paired, non-normal distribution), p-adjustment using the Bonferroni method, and a paired t-test. If three timepoints were compared, the Friedman test was performed, if possible, followed by pairwise comparison using the Wilcoxon test. A value of p < 0.05 was considered to be statistically significant and adjusted for multiple comparisons (using Bonferroni) when performing multiple tests. All comparisons are displayed as medians with a minimum and maximum range; this is stated otherwise if not.
3
Statistical Analysis of Biological Parameters
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Statistical analyses of body weight, BALF parameters, and relative organ weight were conducted by using the StatLight software (Yukms Co., Ltd., Japan). Bartlett’s test for homogeneity of variance was conducted, and when the variances were homogeneous at a significance level of 5%, Dunnett’s test was conducted. When the variances were not homogeneous, Dunnett’s test for nonparametric data was conducted. Statistical significances were judged at the 5% probability level. Regression analyses were performed by using Microsoft Office Excel 2013 and GraphPad Prism 6 version 6.01 (GraphPad Software, Inc., USA) for Windows.
4
Statistical Analysis in Research
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All statistics analyses and calculations were performed using Microsoft Office Excel 2013 and GraphPad Prism software v5.03 (GraphPad Software, San Diego, CA, USA). In this article, statistical significance was defined as a p value <0.05 using t-tests, Mann–Whitney U tests, or Fisher's exact tests as appropriate. All p values are two-tailed.
5
Quantitative Analysis of Nociceptor Responses
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Graphing and statistical analysis was undertaken with a combination of Microsoft Office Excel 2013 and GraphPad Prism (Version 8). All data sets were checked for normality using Kolmogorov-Smirnov tests. Details of statistical tests and sample sizes are recorded in the appropriate figure legends.
Main effects from ANOVAs are expressed as an F-statistic and P-value within brackets. All data plotted represent mean ± SEM. Statistical differences between groups in in vivo imaging experiments involving different drug groups, neuron sizes and doses were compared using a 3-way repeated measures analysis of variance (ANOVA) with Sidak's post hoc comparisons. For imaging experiments comparing drug effects on propagation in heat responsive nociceptors, statistical differences were determined using a 2-way repeated measures ANOVA with Sidak's post hoc comparisons. For imaging experiments involving muscle afferents, statistical differences between groups were determined using a 2-way ANOVA with Tukey's post hoc comparison. The proportion of neurons blocked by TTX in different stimuli response groups was compared using Chi-squared test. For analysis of the in vivo electrophysiology data, conduction velocities were compared between models using Kruskal-Wallis tests and the proportion of neurons blocked between drug groups was compared using Fishers exact test.
Main effects from ANOVAs are expressed as an F-statistic and P-value within brackets. All data plotted represent mean ± SEM. Statistical differences between groups in in vivo imaging experiments involving different drug groups, neuron sizes and doses were compared using a 3-way repeated measures analysis of variance (ANOVA) with Sidak's post hoc comparisons. For imaging experiments comparing drug effects on propagation in heat responsive nociceptors, statistical differences were determined using a 2-way repeated measures ANOVA with Sidak's post hoc comparisons. For imaging experiments involving muscle afferents, statistical differences between groups were determined using a 2-way ANOVA with Tukey's post hoc comparison. The proportion of neurons blocked by TTX in different stimuli response groups was compared using Chi-squared test. For analysis of the in vivo electrophysiology data, conduction velocities were compared between models using Kruskal-Wallis tests and the proportion of neurons blocked between drug groups was compared using Fishers exact test.
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