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Fluo 8 calcium assay

Manufactured by Abcam
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Fluo-8 calcium assay is a fluorescent dye used for measuring intracellular calcium levels in cells. It binds to calcium ions, resulting in an increase in fluorescence intensity that can be detected and quantified. The assay provides a simple and sensitive method for monitoring calcium signaling events in live cells.

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Lab products found in correlation

Flexstation 2, by Molecular Devices (1 mentions) 96 well plate, by Corning (1 mentions)

Spelling variants of this product

Fluo-8 (18 citations) Fluo-8 calcium flux assay kit (14 citations) Fluo-8 No Wash Calcium Assay kit (10 citations) Fluo-8 calcium assay kit (2 citations) Fluo-8 Calcium Flux Assay (2 citations)

2 protocols using fluo 8 calcium assay

1

Histamine-Induced Calcium Signaling in HUVECs

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WT HUVECs, AD-HIES HUVECs, and MLE cells were seeded at 20,000/well in a 96 well plate (Corning, Tewksbury, MA) and incubated overnight. A Fluo-8 calcium assay was then performed (Abcam, Cambridge, MA). Cells were washed in PBS and 100ul of Fluo-8 dye-loading solution was added to each well. Plates were incubated at 37°C for 30 minutes and then 30 minutes at room temperature. Histamine was added (1 µM, 10 µM, 100 µM, and 1000 µM) and fluorescent intensity was monitored at Ex/Em=490/525 nm using a Flexstation II (Molecular Devices, Sunnyvale CA).
Hox V., O’Connell M.P., Lyons J.J., Sackstein P., Dimaggio T., Jones N., Nelson C., Boehm M., Holland S.M., Freeman A.F., Tweardy D.J., Olivera A., Metcalfe D.D, & Milner J.D. (2016). Diminution of STAT3 signaling inhibits vascular permeability and anaphylaxis. The Journal of allergy and clinical immunology, 138(1), 187-199.
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2

Intracellular ATP and Calcium Assays

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ATP in virus-treated cells was measured using the Intracellular ATP kit HS (Biothema) by the manufacturer’s instructions. Samples were analyzed by luminometry, normalized for protein, and expressed as percentage of controls. Intracellular calcium levels were measured using the fluo-8 calcium assay (AbCam). The dye was added to cells and incubated for 1 hr, and then fluorescence was measured at excitation/emission of 490/525 nm.
Dyer A., Di Y., Calderon H., Illingworth S., Kueberuwa G., Tedcastle A., Jakeman P., Chia S.L., Brown A., Silva M.A., Barlow D., Beadle J., Hermiston T., Ferguson D.J., Champion B., Fisher K.D, & Seymour L.W. (2016). Oncolytic Group B Adenovirus Enadenotucirev Mediates Non-apoptotic Cell Death with Membrane Disruption and Release of Inflammatory Mediators. Molecular Therapy Oncolytics, 4, 18-30.
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