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Fb 12

Manufactured by Merck Group
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The FB-12 is a laboratory equipment product manufactured by Merck Group. It is designed to perform a specific core function, but a detailed description while maintaining an unbiased and factual approach is not available.

Automatically generated - may contain errors

Lab products found in correlation

Collagenase type 1, by Thermo Fisher Scientific (1 mentions) D8418, by Merck Group (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Hmc3 cells, by American Type Culture Collection (1 mentions)

2 protocols using fb 12

1

Reduction Mammoplasty Tissue Acquisition

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Anonymous reduction mammoplasty samples were acquired from NCI Cooperative Human Tissue Network (CHTN) and from Department of Surgery, Feinberg School of Medicine, Northwestern University. Other investigators may have received specimens from the same tissue specimens obtained through NCI CHTN. Specimens were anonymized then collected and distributed by CHTN, specimens are covered under collection/distribution of tissues under consent or waiver of consent. Samples were washed in PBS (Corning 21-031-CV) and mechanically dissociated using a razor blade. Dissociated samples were digested overnight in DMEM (Corning 10-013-CV) with Collagenase Type I, 2 mg/mL (Life Technologies 17100-017). Viable organoids were separated using differential centrifugation and viably frozen in 50% FBS (Omega Scientific FB-12), 40% DMEM, and 10% DMSO (Sigma-Aldrich D8418) by volume.
Nguyen Q.H., Pervolarakis N., Blake K., Ma D., Davis R.T., James N., Phung A.T., Willey E., Kumar R., Jabart E., Driver I., Rock J., Goga A., Khan S.A., Lawson D.A., Werb Z, & Kessenbrock K. (2018). Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity. Nature Communications, 9, 2028.
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2

Maintenance of HEK-293, HMC3, and BV-2 Cells

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Human Embryonic Kidney (HEK)-293 cells, and HMC3 cells, originally obtained from ATCC (Manassas, VA, USA), were maintained in growth media consisting of Dulbecco’s Modified Eagles Medium (DMEM) (Corning Life Sciences, Cellgro #10–013-CV; or Cytiva Hyclone, Marlborough, MA, USA, #SH30243.02), 10% FBS (Omega Scientific, Tarzana, CA, USA, #FB-12; or Sigma, St. Louis, MO, USA, # 12306C), 1% penicillin (10,000 units)/streptomycin (10 mg) (Pen/Strep, Gibco from Thermo Fisher Scientific, Waltham, MA, USA, #15140122) and 1% L-glutamine (200 mM) (Gibco #25030081). BV-2 cells were maintained in DMEM media containing 10% FBS as above.
Finneran D., Li Q., Subbarayan M.S., Joly-Amado A., Kamath S., Dengler D.G., Gordon M.N., Jackson M.R., Morgan D., Bickford P.C., Smith L.H, & Nash K.R. (2022). CONCENTRATION AND PROTEOLYSIS OF CX3CL1 MAY REGULATE THE MICROGLIAL RESPONSE TO CX3CL1. Glia, 71(2), 245-258.
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