The nitrocellulose membranes were blocked and antibodies were applied in 5% milk in TRIS-buffered saline/Tween-20 (TBST, 0.1M TRIS-HCl, 0.9% [w/v] NaCl, 0.1% [v/v] Tween-20, pH 7.5). The antibodies used were anti-NF45/ILF2 (Bethyl, A303-147A-M), anti-DHX9 (Bethyl, A300-855A-M), anti-Matrin-3 (Bethyl, A300-591A-M), anti-hnRNPA1 (Novus, NB100-672), anti-Caprin-1 (Proteintech, 15112-1-AP), anti-GST (Santa Cruz, sc-459), anti-DDX3X (Sigma, HPA001648), anti-actin (Santa Cruz, sc-1616), anti-FLAG (Sigma, A8592), anti-Alix (Santa Cruz, sc-49268), anti-TSG101 (GeneTex, GTX70255), anti-CD63 (Santa Cruz, sc-15363), and anti-Histone H3 (Cell Signaling Technology, 9715). The immunoblotting images were acquired with the Chemidoc MP Imaging System (Bio-Rad) and quantified with the Image Lab software (Bio-Rad).
Anti hnrnpa1
Manufactured by Novus Biologicals
Anti-hnRNPA1 is a laboratory reagent used for the detection and quantification of the heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) protein. hnRNPA1 is a nuclear protein involved in the packaging and processing of pre-mRNA.
Automatically generated - may contain errors
Lab products found in correlation
Anti flag, by Merck Group (2 mentions)
Anti matrin 3, by Fortis Life Sciences (1 mentions)
Anti caprin 1, by Proteintech (1 mentions)
Anti tsg101, by GeneTex (1 mentions)
Anti alix, by Santa Cruz Biotechnology (1 mentions)
Anti histone h3, by Cell Signaling Technology (1 mentions)
Anti gst, by Santa Cruz Biotechnology (1 mentions)
Anti cd63, by Santa Cruz Biotechnology (1 mentions)
Anti actin, by Santa Cruz Biotechnology (1 mentions)
Image lab software, by Bio-Rad (1 mentions)
Chemidoc mp imaging system, by Bio-Rad (1 mentions)
Anti ha, by Abcam (1 mentions)
Mab374, by Merck Group (1 mentions)
Anti mbp, by New England Biolabs (1 mentions)
Anti hnrnp h, by Fortis Life Sciences (1 mentions)
Ddx17, by Fortis Life Sciences (1 mentions)
Hnrnp f, by Santa Cruz Biotechnology (1 mentions)
Anti gapdh, by Merck Group (1 mentions)
Anti rna pol 2, by Santa Cruz Biotechnology (1 mentions)
Anti hnrnp m, by Santa Cruz Biotechnology (1 mentions)
2 protocols using anti hnrnpa1
1
Western Blot Antibody Validation
2
Antibody Sourcing and Characterization
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Antibodies were obtained commercially. From Santa Cruz Biotechnology: anti-AKAP95 (sc-10766, 1:500); anti-DDX5 (sc-32858, 1:500); anti-hnRNP M (sc-20002, 1:500); hnRNP F (sc-10045, 1:500); anti-His (sc-803, 1:500); and anti-RNA Pol II (sc-899, 1:500). From others: Anti-hnRNP A1 (Novus Biologicals, NB100–672, 1:2,000), anti-Pol II (Covance, MPY-127R for clones 8WG16, H14 and H5, 1:500), anti-hnRNP H (Bethyl Laboratories, A300–511A, 1:2,000); DDX17 (Bethyl Laboratories, A300–509A, 1:2,000); anti-HA (Abcam, ab9110, 1:1,000); anti-GAPDH (Chemicon, MAB374, 1:5,000); anti-FLAG (Sigma, A8592, 1:1,000); anti-FLAG (Sigma, A2220, M2 beads); and anti-MBP (New England BioLabs, E8032S, 1:5,000).
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