Ht7700 exalens microscope

The particle size, polydispersity index and zeta potential were determined by dynamic and electrophoretic light scattering on Anton Paar LiteSizer 500 (Graz, Austria) device and Calliope software. The data were obtained in a series of 3 measurements; the measurement angle was 175 degrees and undiluted samples were used. Correlograms obtained with DLS are provided in the Section S2 of the Supplementary Materials.
Transmission electron microscopy (TEM) images were obtained at the interdisciplinary center “Analytical microscopy” of Kazan Federal University, using a Hitachi HT7700 Exalens microscope (Tokyo, Japan). The images were acquired at an accelerating voltage of 100 kV. Samples were dispersed on 300 mesh 3 mm copper grids (Ted Pella, Redding, CA, USA) with continuous carbon-formvar support films.

Manganese chloride tetrahydrate (99%), oleic acid (tech, 90%) and 1-Octadecene (tech, 90%) were purchased from Sigma-Aldrich (USA). Sodium oleate was purchased from Sinopharm Chemical Reagent Co., Ltd. All reagents were used without further purification.
Transmission electron microscopy (TEM) and the related high-resolution TEM (HRTEM) images were performed on a FEI Tecnai G2 F20 microscope (accelerating voltage, 200 kV) and a Hitachi HT7700 Exalens microscope (accelerating voltage, 120 kV). The X-ray powder diffraction (XRD) patterns of the nanoparticles were acquired on a D/MAX-Ultima VI X-ray powder diffractometer (Rigaku Co., Japan). The X-ray absorption spectra (XPS) were conducted at an Escalab 250Xi X-ray photoelectron spectrometer (Thermo Scientific). The hysteresis loops at 300 K were recorded by the superconducting quantum interference device (SQUID). Dynamic light scattering (DLS) were measured by Zetasizer Nano ZS (Malvern Instruments Ltd., England). The measurement of relaxivity and phantom imaging at 0.5 T were all performed on an NMI20-Analyst system. In vivo MRI were performed on 7 T micro MRI System. The concentrations of metals were measured by inductively coupled plasma mass spectroscopy (ICP-MS) on an iCAP RQ system (Thermo Fisher).

TEM images were obtained at the Interdisciplinary Center for Analytical Microscopy of Kazan (Volga Region) Federal University, using a Hitachi HT7700 Exalens microscope, (Hitachi, Tokyo, Japan). The images were acquired at an accelerating voltage of 100 kV. Samples were dispersed on 300 mesh 3 mm copper grids (Ted Pella) with continuous carbon-formvar support films. To exclude transfersome damage, 5 μL of the solution was dried at room temperature.

TEM analysis was carried out according to the literature [20 (link)] with the Hitachi HT7700 Exalens microscope (Tokyo, Japan).

The mean particle size, zeta potential and polydispersity index were determined by dynamic light scattering (DLS), using the Malvern Instrument Zetasizer Nano (Worcestershire, UK). The measured autocorrelation functions were analyzed by Malvern DTS software, applying the second-order cumulant expansion methods. The size (hydrodynamic diameter, nm) was calculated according to the Einstein-Stokes relationship D = kBT/3πηx, in which D is the diffusion coefficient, kB the Boltzmann’s constant, T the absolute temperature, η the viscosity, and x the hydrodynamic diameter of nanoparticles. The diffusion coefficient was determined at least in triplicate for each sample. The average error of measurements was approximately 4%. All samples were diluted with ultra-purified water to suitable concentration and analyzed in triplicate.
Transmission electron microscopy (TEM) images were obtained using a Hitachi HT7700 Exalens microscope, Japan. The images were acquired at an accelerating voltage of 100 keV. Samples were dispersed on 300 mesh 3 mm copper grids (Ted Pella) with continuous carbon formvar support films.

The disks containing six SPEs, a PET substrate, and a polydimethylsiloxane (PDMS) insulating loop were obtained from Weihai Poten Technology Co., Ltd. (Weihai, China). The six SPEs included four carbon electrodes as the working electrodes, Ag/AgCl as the reference electrode, and a carbon electrode as the counter electrode. DPV was performed using a BIOSYS p15e max biosensor system from Shenzhen Refresh Biosensing Technology Co., Ltd. (Shenzhen, China). This hand-held electrochemical station was driven by a smartphone installed with an application that uses Blue tooth. CV and EIS were conducted using a CHI660E electrochemical station (Chenhua, Shanghai, China). The morphologies and energy-dispersive X-ray (EDX) patterns of MOFs and MB@MOFs were obtained through high-resolution transmission electron microscopy (HRTEM) using an HT7700 Exalens microscope (Hitachi, Tokyo, Japan) at an accelerating voltage of 120 kV. X-ray diffraction (XRD) patterns were obtained using a D2 PHASER diffractometer (BRUKER, Mannheim, Germany). Adsorption–desorption isotherms were assessed using an automated surface area and porosity analyzer (Quantachrome, Boynton Beach, FL, USA).