Female 8 week old nude mice (Charles River) were used for all microPET and biodistribution studies. All procedures performed on the animals were approved by the University’s Institutional Animal Care and Use Committee (APLAC #29179), and were within the guidelines of humane care of laboratory animals.
Female 8 week old nude mice
Manufactured by Charles River Laboratories
Sourced in United States
Female 8 week old nude mice are an inbred strain of laboratory mice that lack a functional immune system. They are commonly used as animal models in biomedical research for the study of cancer, infectious diseases, and the evaluation of therapeutic interventions.
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Lab products found in correlation
2 protocols using female 8 week old nude mice
1
Nude Mice for MicroPET and Biodistribution
2
Breast Cancer Xenograft Tumor Models
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Female 8-week-old nude mice (Charles River, Wilmington, MA, USA) were used for all tumor inoculation studies. All procedures performed on the animals were approved by the University’s Institutional Animal Care and Use Committee (APLAC# 30018/20786) and were within the guidelines of humane care of laboratory animals. Various breast cancer cell lines, as listed in Table 1 , were cultured under standard conditions along with the positive and negative-expressing CD47 DLD1 cell lines. The xenograft tumor models were generated by subcutaneous injection of 5 million cells in 50 µL HBSS mixed with 50 uL of Matrigel on the flanks of the mice. Tumors were then harvested for SERS NP staining and Raman imaging from euthanized mice when the tumor volume reached approximately 200–300 mm3.
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