Dapi 2 4 amidinophenyl indole 6 carboxamide dihydrochloride

PLL (Molecular weight = 120 kDa, ~574 lysine unit, one HBr per lysine residual) was procured from Polysciences (Warrington, PA, USA). Trisodium citrate dihydrate was procured from the Merck (Darmstadt, Germany). Sodium phosphate dibasic heptahydrate, fluoromount mounting media, and ICG were purchased from the Sigma Aldrich (St. Louis, MO, USA) and were used as received without further purification. Stock solutions of the ICG and salts were prepared in de-ionized (DI) water (18.2 MΩ Millipore, Sartorius system). All the prepared stock solutions were stored at 4 °C. Cancerous cells were procured from the National Centre for Cell Science (NCCS) Pune, India. Fetal Bovine Serum (FBS), Dulbecco’s Modified Eagle Medium (DMEM), 0.25% trypsin-1 mM ethylenediaminetetraacetic acid (EDTA), penicillin-streptomycin, and 2.5% trypsin without phenol red were purchased from Gibco (Thermo Fisher Scientific Inc., India). Colorimetric assay (MTT, (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide)) was procured from Himedia chemicals (India). For nucleus staining, DAPI (2-(4-amidinophenyl) indole-6-carboxamide-dihydrochloride) was procured from the Tokyo Chemical Industry (TCI, India).