Anti hla dr pc7

Manufactured by Beckman Coulter

Sourced in United States, France

The Anti-HLA-DR-PC7 is a fluorescent-labeled antibody designed for flow cytometric detection of HLA-DR expression on human cells. It is a key component in various immunological assays and research applications.

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4 protocols using anti hla dr pc7

Isolating Ma-Mel-36 Subpopulations by Flow Cytometry

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The following directly labelled antibodies were used for staining of cellular surface markers: anti-HLA-ABC-APC (eBiosciences, clone W6/32; 1 μl), anti-CD54-PE (Beckmann Coulter, clone 84H10; 2.5 μl), anti-PD-L1-PE (Biolegend, clone 29E2A3; 5 μl) and anti-HLA-DR-PC7 (Beckmann Coulter, clone Immun-357; 2.5 μl). After fixation, stained cells were analysed by flow cytometry on a Gallios flow cytometer (Beckmann Coulter) and Kaluza (Beckman Coulter) software, respectively, for data analysis. In order to isolate specific Ma-Mel-36 subpopulations, cells were stained with anti-HLA-DR-PC7 and sorted based on the specific expression of the surface markers by flow cytometry on an Aria II cell sorter and the FACS Diva software (BD Biosciences).

Sucker A., Zhao F., Pieper N., Heeke C., Maltaner R., Stadtler N., Real B., Bielefeld N., Howe S., Weide B., Gutzmer R., Utikal J., Loquai C., Gogas H., Klein-Hitpass L., Zeschnigk M., Westendorf A.M., Trilling M., Horn S., Schilling B., Schadendorf D., Griewank K.G, & Paschen A. (2017). Acquired IFNγ resistance impairs anti-tumor immunity and gives rise to T-cell-resistant melanoma lesions. Nature Communications, 8, 15440.

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Flow Cytometry of Lymphocyte Subsets

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Anti-CD3-FITC, anti-CD16-PC5, anti-CD56-PE, anti-CD19-ECD, anti-HLA-DR-PC7,
anti-CD45-FITC, anti-CD4-PE, anti-CD3-PC5, and anti-CD8-ECD fluorescent
conjugated monoclonal antibodies (Beckman Coulter, USA) were used for
quantification of major lymphocyte subsets.

Adas G., Cukurova Z., Yasar K.K., Yilmaz R., Isiksacan N., Kasapoglu P., Yesilbag Z., Koyuncu I, & Karaoz E. (2021). The Systematic Effect of Mesenchymal Stem Cell Therapy in Critical COVID-19 Patients: A Prospective Double Controlled Trial. Cell Transplantation, 30, 09636897211024942.

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Flow Cytometric Phenotyping of Dendritic Cells

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Before maturation (0 h) and after 6 h and 24 h of maturation, the harvested DCs were washed. Staining was performed with fluorescent antibody: anti-HLA-DR-PC7, anti-CD80-FITC, anti-CD86-PE (Beckman Coulter Inc., Immunotech, France), and anti-CD83-APC (BD Pharmingen, BD Bioscience, NJ, USA). The DCs were measured using a FACSCanto flow cytometer (Becton Dickinson, NJ, USA). Data were analyzed using BD FACSDiva 6.0 software.

Stumpfova Z., Hezova R., Meli A.C., Slaby O, & Michalek J. (2014). MicroRNA Profiling of Activated and Tolerogenic Human Dendritic Cells. Mediators of Inflammation, 2014, 259689.

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MDSC and T-cell Phenotyping using Flow Cytometry

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Evaluation of MDSC percentage was performed using dry preformulated antibody panels DuraClone (anti-CD11b FITC, anti-CD33 PC7, anti-CD14 PC5.5, anti-human leucocyte antigen locus DR (HLA-DR) Phycoerythrin-Texas Red conjugate, energy coupled dye, cocktail of antibodies anti-CD3 APC-A750, -CD56 APC A750, -CD19 APC A750 (Lin), anti-CD15 pacific blue, and CD45 krome orange, DRAQ-7) from Beckman Coulter. See Figure S1, Supplemental Digital Content 2, https://links.lww.com/QAI/C170 for gating strategy used to identify MDSC.
T-cell phenotype was accomplished using dry preformulated antibody panels DuraClone (anti-CD3 APC-A750, anti-CD4 APC, anti-CD197 PE, anti-CD45RA Phycoerythrin-Texas Red conjugate, energy coupled dye, anti-HLA-DR PC7, anti-CD38 pacific blue, anti-CD45 krome orange) from Beckman Coulter. Single staining and compensation controls were used to set up flow cytometry experiments. Acquisition of 100,000 events was performed in the leukocyte-gated population on Navios flow cytometer and analyzed with Kaluza software (Beckman Coulter).

Grassi G., Notari S., Cicalini S., Casetti R., Cimini E., Bordoni V., Gagliardini R., Mazzotta V., Antinori A., Agrati C, & Sacchi A. (2024). Brief Report: In cART-Treated HIV-Infected Patients, Immunologic Failure Is Associated With a High Myeloid-Derived Suppressor Cell Frequency. Journal of acquired immune deficiency syndromes (1999), 95(2).

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