Polyclonal anti cdc20

The following antibodies were purchased from Santa Cruz: monoclonal anti-HA, rabbit polyclonal anti-HA and polyclonal anti-Cdc20. Rabbit polyclonal antibodies to Histone H3, Cdc27 and PHistone-H2AX (Ser139) antibodies were purchased from Cell Signalling Technologies. The following antibodies were purchased from Sigma-Aldrich: monoclonal anti-g-tubulin, polyclonal α-tubulin, HRP-conjugated goat anti-mouse, HRP-conjugated goat anti-rabbit. A human anti-centromere antibody was purchased from Europa Bioproducts Ltd. Monoclonal antibodies to PCNA, Mad2B and c-myc were purchased from BD Transduction Laboratories. The following antibodies were purchased from Invitrogen: Alexa Fluor 488 goat anti-mouse IgG, Alexa Fluor 488 goat anti-rabbit IgG, Alexa Fluor 594 rabbit anti-mouse IgG and Alexa Fluor 594 goat anti-human IgG.

The following antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA): monoclonal anti-HA, rabbit polyclonal anti-HA and polyclonal anti-Cdc20. Rabbit polyclonal antibodies to Chk1, PChk1 (Ser317), Cdc27, PHistone-H2AX (Ser139), Histone H3 antibodies and mouse monoclonal FLAG antibody were purchased from Cell Signaling Technology (Beverly, MA, USA). The following antibodies were purchased from Sigma-Aldrich (St Louis, MO, USA): monoclonal anti-γ-tubulin, polyclonal α-tubulin, horseradish peroxidase (HRP)-conjugated goat anti-mouse, HRP-conjugated goat anti-rabbit. A human anti-centromere antibody was purchased from Europa Bioproducts Ltd (Cambridge, UK). Monoclonal antibodies to Mad2B, Mad2A, PCNA and c-myc were purchased from BD Transduction Laboratories (Heidelberg, Germany). The following antibodies were purchased from Invitrogen (Carlsbad, CA, USA): Alexa Fluor 488 goat anti-mouse IgG, Alexa Fluor 488 goat anti-rabbit IgG, Alexa Fluor 594 rabbit anti-mouse IgG and Alexa Fluor 594 goat anti-human IgG.