Streptavidin biotin complex kit

After dewaxing and hydration of paraffin sections, the liver and cortex tissues were stained with hematoxylin and eosin (H&E). The sections of frozen liver tissue were stained with Oil Red O (ORO). Nissl staining was performed on the OFC samples for Nissl body observation. The OFC was also stained with anti-brain-derived neurotrophic factor (BDNF, sc-33904), anti-Sox-2 (sc-365964), and anti-ionized calcium-binding adapter molecule 1 (IBA-1, sc-32725; Santa Cruz Biotechnology, Santa Cruz, CA, United States). Five fields on each slide were randomly selected, viewed under a fluorescence microscope (Nikon TE2000-U, Nikon, Japan), and analyzed using Image Pro-Plus 6.0 software (Media Cybernetics, Silver Spring, MD, United States). The minimal pixel number was set at 50 pixels. The average and cumulative optical density values, average area, and average diameter were analyzed. StreptAvidin Biotin Complex kits were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Antibodies against Type I and Type III collagen, Strept Avidin Biotin Complex kits and SP-9,000 kits were purchased from Boster Biological Technology, Ltd. (Wuhan, China). Results were analyzed by randomly selecting five fields of vision (magnification, ×400) for each specimen. Five images of each vision were recorded.

Sections were blocked for 1hr at room temperature in 10% goat serum and 0.1% Triton. Sections were then incubated with anti-c-Abl (Santa Cruz, sc-887, 1:100), p-ERK antibodies (CST, 9106S,1:200), Ki67 (Abcam, ab15580, 1:150), CD3 (Abcam, ab16669, 1:100), αSMA (Sigma, A5228, 1:200) overnight at 4 °C, and incubated with secondary antibodies (Life, A-11001/A-11034, 1:100) for 30 min at 37 °C for Immunofluorescence staining, or incubated with Streptavidin Biotin Complex kit (BOSTER, SA1050) for immunohistochemistry staining.