Glucose uptake fluorometric assay kit

Manufactured by Merck Group

Sourced in United States

The Glucose Uptake Fluorometric Assay Kit is a laboratory equipment product designed to measure the uptake of glucose in cells. The assay utilizes a fluorometric detection method to quantify the amount of glucose taken up by cells under various experimental conditions.

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Lab products found in correlation

Myriocin, by Merck Group (1 mentions) Oleate, by Merck Group (1 mentions) Ceranib 2, by Merck Group (1 mentions) A939572, by Merck Group (1 mentions) Insulin, by Merck Group (1 mentions) Lactate assay kit, by Merck Group (1 mentions) Atp assay kit, by Merck Group (1 mentions)

Close spelling variants of this product

Fluorometric Assay Kit Glucose assay kit Fluorometric kits

6 protocols using glucose uptake fluorometric assay kit

Quantifying Tumor Glucose Uptake and Lactate

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Relative glucose uptake required by tumor cells was measured by Glucose Uptake Fluorometric Assay Kit (MAK084, Sigma-Aldrich, USA), and relative lactate production was measured by Lactic Acid Content Assay Kit (D799851-0050, Sangon, China) according to the technical bulletin provided by manufacturer.

Cai J., Cui Z., Zhou J., Zhang B., Lu R., Ding Y, & Hu H. (2022). METTL3 promotes glycolysis and cholangiocarcinoma progression by mediating the m6A modification of AKR1B10. Cancer Cell International, 22, 385.

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Insulin-Dependent Glucose Uptake Assay

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To measure the insulin dependent glucose up-take on explants we have used the Glucose up-take Fluorometric Assay Kit from SIGMA-ALDRICH (MAK084) following the manufacturer’s instructions.

Drareni K., Ballaire R., Barilla S., Mathew M.J., Toubal A., Fan R., Liang N., Chollet C., Huang Z., Kondili M., Foufelle F., Soprani A., Roussel R., Gautier J.F., Alzaid F., Treuter E, & Venteclef N. (2018). GPS2 Deficiency Triggers Maladaptive White Adipose Tissue Expansion in Obesity via HIF1A Activation. Cell Reports, 24(11), 2957-2971.e6.

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Biochemical Analysis of Insulin Signaling

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All reagent-grade chemicals including insulin, TOFA, FB1, myriocin, ceranib-2, A939572, oleate, and stearate were obtained from Sigma-Aldrich. C2-cer was obtained from Merck Chemicals. C-75 was from Tocris Bioscience. Antibodies against ⁴⁷³Ser-Akt, ³⁰⁸Thr-Akt, native PKB/Akt, GLUT4, and α1 NA-K-ATPase were from Cell Signaling Technology, and antibody against ceramide (MID 15B4 clone) was from Enzo Life Sciences. Horseradish peroxidase anti-rabbit and horseradish peroxidase anti-mouse were from Jackson ImmunoResearch Laboratories, and the enhancer chemiluminescent Supersignal was from Thermo Fisher Scientific. Ceramide secondary antibody (Alexa fluor 488 goat anti-mouse) was from Thermo Fisher Scientific. Glucose uptake fluorometric assay kit was from Sigma-Aldrich.

Bandet C.L., Tan-Chen S., Ali-Berrada S., Campana M., Poirier M., Blachnio-Zabielska A., Pais-de-Barros J.P., Rouch C., Ferré P., Foufelle F., Le Stunff H, & Hajduch E. (2023). Ceramide analog C2-cer induces a loss in insulin sensitivity in muscle cells through the salvage/recycling pathway. The Journal of Biological Chemistry, 299(6), 104815.

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Metabolic Effects of SUN2 Modulation

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The glucose uptake, lactate secretion and ATP level of HSC-6 and SCC-9 cells upon SUN2 overexpression or knockdown, were detected using Glucose Uptake Fluorometric Assay Kit, Lactate Assay Kit and ATP Assay Kit (Sigma, St. Louis, MO, USA) respectively based on the procedure of manufacturers.

Liao Y., Zhao T., Li L.Y, & Wang F.Q. (2020). Elevated Sad1 and UNC84 Domain Containing 2 (SUN2) level inhibits cell growth and aerobic glycolysis in oral cancer through reducing the expressions of glucose transporter 1 (GLUT1) and lactate dehydrogenase A (LDHA). Journal of Dental Sciences, 16(1), 460-466.

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Glucose Uptake in EpiWAT Explants

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EpiWAT explants were processed to measure glucose uptake with glucose analog 2-DG. After starvation and 2-DG uptake, explants were lysed in an extraction buffer. Lysates were processed according to the manufacturer’s protocol (Glucose Uptake Fluorometric Assay Kit, MAK084, Sigma–Aldrich).

Orliaguet L., Ejlalmanesh T., Humbert A., Ballaire R., Diedisheim M., Julla J.B., Chokr D., Cuenco J., Michieletto J., Charbit J., Lindén D., Boucher J., Potier C., Hamimi A., Lemoine S., Blugeon C., Legoix P., Lameiras S., Baudrin L.G., Baulande S., Soprani A., Castelli F.A., Fenaille F., Riveline J.P., Dalmas E., Rieusset J., Gautier J.F., Venteclef N, & Alzaid F. (2022). Early macrophage response to obesity encompasses Interferon Regulatory Factor 5 regulated mitochondrial architecture remodelling. Nature Communications, 13, 5089.

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Insulin-stimulated 2DG Uptake in NDM Adipocytes

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NDM adipocytes were serum starved for 3 hours, followed by glucose starvation in KRPH buffer supplemented with 2% BSA for 40 mins. Then cells were treated with 1μM insulin for 20 minutes, followed by 10 mM 2-deoxy glucose 6 phosphate (2DG6P). Glucose uptake was performed using Glucose Uptake Fluorometric assay kit (Sigma # MAK084). A standard curve with 2DG6P was generated on the same plate. Fluorescence was measured at Ex 535 nm and Em 587 nm and 2-deoxy glucose uptake of samples was calculated from the standard curve.

Tong H.H., Blue L.E., James M.A., Chen Y.P, & DeMaria T.F. (2000). Evaluation of Phase Variation of Nontypeable Haemophilus influenzae Lipooligosaccharide during Nasopharyngeal Colonization and Development of Otitis Media in the Chinchilla Model. Infection and Immunity, 68(8), 4593-4597.

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