All solvents and reagents used in this study were of analytical quality. Acetonitrile (ACN), methanol (MeOH), acetic acid (C2H4O2−) and ammonia (NH4) were purchased from Honeywell (Bucharest, Romania). Ultra-pure water (H2O) was purchased from VWR (Fontenay-sous-Bois, France); Chloroform (CHCl3) was purchased from Carlo Erba Reactifs (SDS, Peypin, France). Ammonium Fluoride (AF) was purchased from Acros Organics (Geel, Belgium). Metabolomics isotope labeled internal standards (l -Leucine-5,5,5-d3, l -Tryptophan-2,3,3-d3, Indole-2,4,5,6,7-d5-3-acetic acid and 1,14-Tetradecanedioic-d24 acid) were from Sigma–Aldrich (Saint Quentin Fallavier, France) and CDN Isotopes (Pointe-Claire, QC, Canada). MSCAL6 ProteoMass LTQ/FT-Hybrid, standard mixtures used for calibration of the MS instrument, were obtained from Sigma–Aldrich (Saint Quentin Fallavier, France).
Indole 2 4 5 6 7 d5 3 acetic acid
Manufactured by Merck Group
Sourced in France
Indole-2,4,5,6,7-d5-3-acetic acid is a labeled compound used in research and analysis. It contains a deuterium-labeled indole ring structure and a carboxylic acid group. The core function of this compound is to serve as a labeled standard or intermediate in various analytical and research applications.
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Lab products found in correlation
2 protocols using indole 2 4 5 6 7 d5 3 acetic acid
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Analytical-grade solvents and reagents
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Metabolomics Protocol with Isotope Standards
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Reagents and solvents were of LC-MS grade quality. Acetonitrile and acetic acid were purchased from Honeywell Chromasolv (Bucharest, Romania) and water from VWR (Fontenay-sous-Bois, France).
Isotope-labeled internal standards, namely, L-leucine-5,5,5-d3, L-tryptophan-2,3,3-d3, indole-2,4,5,6,7-d5-3-acetic acid, and 1,14-tetradecanedioic-d24 acid were purchased from Sigma-Aldrich (Saint Quentin Fallavier, France) and CDN Isotopes (Québec, Canada).
Pure chemical standards for targeted MS2 confirmation were from Sigma-Aldrich and Acros Organic. MSCAL6 ProteoMass LTQ/FT-Hybrid, standard mixtures used for calibration of the MS instrument (positive and negative ionization mode) were obtained from Sigma-Aldrich (Saint Quentin Fallavier, France).
Isotope-labeled internal standards, namely, L-leucine-5,5,5-d3, L-tryptophan-2,3,3-d3, indole-2,4,5,6,7-d5-3-acetic acid, and 1,14-tetradecanedioic-d24 acid were purchased from Sigma-Aldrich (Saint Quentin Fallavier, France) and CDN Isotopes (Québec, Canada).
Pure chemical standards for targeted MS2 confirmation were from Sigma-Aldrich and Acros Organic. MSCAL6 ProteoMass LTQ/FT-Hybrid, standard mixtures used for calibration of the MS instrument (positive and negative ionization mode) were obtained from Sigma-Aldrich (Saint Quentin Fallavier, France).
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