Apc anti mouse ly 6g antibody

Manufactured by BioLegend

The APC anti-mouse Ly-6G Antibody is a flow cytometry reagent used for the identification and enumeration of mouse Ly-6G-positive cells. Ly-6G is a cell surface marker expressed on neutrophils. The antibody is conjugated to the fluorescent dye Allophycocyanin (APC), providing a convenient means for detection and analysis of Ly-6G-expressing cells by flow cytometry.

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Lab products found in correlation

Brilliant violet 421 anti mouse human cd11b antibody, by BioLegend (1 mentions) Red blood cell lysis buffer, by Solarbio (1 mentions) Percp cyanine5.5 anti mouse cd45 antibody, by BioLegend (1 mentions) Fitc anti mouse human cd11b antibody, by BioLegend (1 mentions) Multi tissue dissociation kit 2, by Miltenyi Biotec (1 mentions) Zombie aqua fixable viability kit, by BioLegend (1 mentions) Lsrfortessa, by BD (1 mentions) Facsaria, by BD (1 mentions) Pe cy7 anti mouse cd45, by BioLegend (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti p akt, by Cell Signaling Technology (1 mentions) Anti 4e bp1, by Cell Signaling Technology (1 mentions) Anti ps6k, by Cell Signaling Technology (1 mentions) Pe anti mouse f4 80 antibody, by BioLegend (1 mentions) Mouse anti gapdh antibody, by Santa Cruz Biotechnology (1 mentions) Anti mtor, by Cell Signaling Technology (1 mentions) Pacific blue anti mouse human cd11b antibody, by BioLegend (1 mentions) Goat anti cd31 af3628, by R&D Systems (1 mentions) Anti p 4ebp1, by Cell Signaling Technology (1 mentions) Anti s6, by Cell Signaling Technology (1 mentions)

3 protocols using apc anti mouse ly 6g antibody

Neutrophil Isolation and Characterization

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Blood was anticoagulated by 15 g/L EDTA-Na₂ and then centrifuged at 1500 rpm for 5 min to remove plasma. Cell populations were resuspended in precooling PBS and then stained using APC anti-mouse Ly-6 g antibody (BioLegend, Cat. No. 127614, dilution 1:100, San Diego, USA) and Brilliant Violet 421 anti-mouse/human CD11b antibody (BioLegend, Cat. No. 121035, dilution 1:100, San Diego, USA) for 30 min at 4 °C. Red blood cells were removed with Red Blood Cell Lysis Buffer (Solarbio, Cat. No. R1010, Beijing, China). Flow cytometry was performed on the Beckman Coulter platform (CA, USA). The neutrophil proportion was analyzed using Kaluza software (Version 2.1).

Lei Y., Tang L., Liu S., Hu S., Wu L., Liu Y., Yang M., Huang S., Tang X., Tang T., Zhao X., Vlodavsky I., Zeng S., Tang B, & Yang S. (2021). Parabacteroides produces acetate to alleviate heparanase-exacerbated acute pancreatitis through reducing neutrophil infiltration. Microbiome, 9, 115.

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Multiparametric flow cytometry for immune cell profiling

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Single-cell suspensions were prepared by using the multi-tissue dissociation kit 2 (Miltenyi Biotec). For cytometric analyses, the cells were incubated with a mixture of antibodies at 4°C for 20 min. The antibodies used in the present study are listed: FITC anti-mouse/human CD11b Antibody (BioLegend) (Cat#101206), PerCP/Cyanine5.5 anti-mouse CD45 Antibody (BioLegend) (Cat#103132), APC anti-mouse Ly-6G Antibody (BioLegend) (Cat#127614), Zombie Aqua Fixable Viability Kit (BioLegend) (Cat# 423102). The obtained results were expressed as the percent or cell number per microgram of tissue. Flow cytometric analysis and cell sorting were performed on an LSRFORTESSA and FACS Aria instruments (BD Biosciences, San Jose, CA, USA) and analyzed using FlowJo software (Tree Star).

Sun X., Chen H., Gao R., Huang Y., Qu Y., Yang H., Wei X., Hu S., Zhang J., Wang P., Zou Y., Hu K., Ge J, & Sun A. (2023). Mitochondrial transplantation ameliorates doxorubicin-induced cardiac dysfunction via activating glutamine metabolism. iScience, 26(10), 107790.

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Detailed Immunoblot Antibody Protocol

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Antibodies used for immunoblot are listed here based on where they are acquired. 1. From Cell Signaling Technology (Danvers, MA): anti-pS6 (4858); anti-pS6K (9204); anti-S6 (2317); anti-p4E-BP1 (2855); anti-4E-BP1(9452); anti-mTOR (2983); anti-pAkt (Ser473) (4060); anti-pAkt (Thr308) (13038); anti-Akt (2920). 2. From BioRad (Hercules, California): anti-CD68 (MCA19571); 3. From Biolegend (San Diego, California): Alexa fluoro-labeled anti-CD 206 (141709); APC anti-mouse Ly-6G Antibody (127614); PE anti-mouse F4/80 Antibody (123110); Pacific Blue™ anti-mouse/human CD11b Antibody (101224); PE/Cy7 anti-mouse CD45 (103114); Brilliant Violet 711™ anti-mouse Ly-6C Antibody (128037); PerCP/Cy5.5 anti-mouse CD206 (MMR) Antibody (141716). 4. From Santa Cruz Biotechnology (Santa Cruz, CA): mouse anti-GAPDH antibody; anti-LAMP1 (sc-20011). 5. From R&D Systems (Minneapolis, MN): goat anti-CD31 (AF3628). 6. From Cedarlane (Burlington, Ontario, Canada): anti-Mouse Mac-3 (CL8993AP).

Chen G., Phan V., Lou X, & Cao D.J. (2021). The mechanistic target of rapamycin complex 1 critically regulates the function of mononuclear phagocytes and promotes cardiac remodeling in acute ischemia. Journal of molecular and cellular cardiology, 159, 62-79.

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