Jasmone

The separation was achieved on a Perkin Elmer Elite-5-MS fused-silica capillary column (30 m × 0.25 mm, film thickness 0.25 µm), using helium as carrier gas at a standard flow rate of 1.1 mL/min. The temperature of the injector was adjusted at 250 °C with an initial temperature of 50 °C, initial hold 5 min, and ramp 4.0 °C/min to 280 °C. The total running time was 62.50 min and the solvent delay was from 0 to 4.0 min. MS scan time was from 4 to 62.5 min, covering mass range 50.00 to 300.00 m/z. The chemical ingredients of the EO were characterized by comparing their mass spectra with the reference spectra in the MS Data Centre of the National Institute of Standards and Technology, and by matching their Kovats and retention indices with values reported in the literature [29 (link),30 (link)]. In addition, the EOs Kovats and retention indices with values were compared with 20% of HPLC grade of reference EOs including α-pinene, eucalyptol, caryophyllene, γ-terpinene, ocimene, endo-borneol, α-terpineol, jasmone, α-cadinene, p-menthane that were purchased from Sigma-Aldrich, Hamburg, Germany) [31 (link)].

All VOC standards were purchased from Sigma-Aldrich (St. Louis, MO, United States): 1-penten-3-ol, 1-penten-3-one, 3-pentanone, α-caryophyllene, α-farnesene, α-pinene, β-caryophyllene, β-farnesene, β-ionone, benzaldehyde, cis-3-hexen-1-ol, cis-3-hexenyl acetate, eugenol, hexyl acetate, jasmone, limonene, linalool, methyl jasmonate, methyl salicylate, ocimene, trans-2-hexen-1-al. All VOC standards were diluted in 100% methanol.