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Dy809

Manufactured by R&D Systems
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Sourced in United Kingdom

The DY809 is a high-performance laboratory centrifuge designed for a variety of applications. It can achieve a maximum speed of 9,000 rpm and a maximum RCF of 12,100 x g. The centrifuge features a microprocessor-controlled system for precise speed and time control. It is equipped with a safety lid lock and an imbalance detection system for safe operation.

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Lab products found in correlation

Duoset elisa, by R&D Systems (1 mentions) Dy720, by R&D Systems (1 mentions) Dy1160, by R&D Systems (1 mentions) Multiskan go plate reader, by Thermo Fisher Scientific (1 mentions)

3 protocols using dy809

1

Quantifying Secreted Osteocalcin and Inflammatory Markers

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Human osteocalcin DuoSet ELISA (R&D Systems; DY1419) was used to measure total secreted osteocalcin in cell culture media. Endothelin Pan Specific, ICAM‐1/CD54, VCAM‐1/CD106, and total MMP‐3 DuoSet ELISAs were performed on cell culture media according to the manufacturer's instructions (Catalog numbers DY1160, DY720, DY809, and DY513; R&D Systems, Abingdon, UK).
Millar S.A., Anderson S.I, & O'sullivan S.E. (2019). Human vascular cell responses to the circulating bone hormone osteocalcin. Journal of Cellular Physiology, 234(11), 21039-21048.
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2

Hemopexin and sVCAM-1 Quantification

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Hemopexin and sVCAM-1 levels were measured in serum by Elisa in accordance with manufacturer’s instructions (Abcam ab171576, Cambridge, UK; and R&D #DY809, Minneapolis, USA, respectively).
Santaterra V.A., Fiusa M.M., Hounkpe B.W., Chenou F., Tonasse W.V., da Costa L.N., Garcia-Weber D., Domingos I.D., de Lima F., Borba-Junior I.T., Araújo A.D., Lucena-Araújo A.R., Bezerra M.A., dos Santos M.N., Costa F.F., Millán J, & De Paula E.V. (2020). Endothelial Barrier Integrity Is Disrupted In Vitro by Heme and by Serum From Sickle Cell Disease Patients. Frontiers in Immunology, 11, 535147.
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3

Angiogenic Secretions in SDF-1α Cultures

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To determine angiogenic growth of the endothelial cultures in SDF-1α GAS, secretion of the soluble forms of endothelial junctional adhesion molecule VE-cadherin (DY938, R&D Systems, UK) and inflammation induced transmembrane molecule VCAM-1 (DY809, R&D Systems, UK) was analyzed. To assay angiogenesis, 100 μl of CM collected from days 3, 7, 10, and 14 were used for the assay and the absorbance was read at 450 nm using a Multiskan GO plate reader (Thermo Scientific, UK). The quantity of soluble VE-cadherin or VCAM-1 released was deduced by calculating against their respective standard curves.
Laiva A.L., O’Brien F.J, & Keogh M.B. (2021). SDF-1α gene-activated collagen scaffold enhances provasculogenic response in a coculture of human endothelial cells with human adipose-derived stromal cells. Journal of Materials Science. Materials in Medicine, 32(3), 26.
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