Siraitia grosvenorii specimens were collected in Guilin, Guangxi Province, China. Xi’an Medical College (Xi’an, Shaanxi) generously donated human breast cancer cells (MDA-MB-231), human hepatoma cells (HepG2) and human non-small cell lung cancer cells (A549). Chlorosulfonic acid, concentrated hydrochloric acid, trifluoroacetic acid, sodium hydroxide and pyridine were obtained from Sinopharm Chemical Reagent Company; Annexin V-FITC apoptosis kit, BCA protein concentration assay kit and reactive oxygen species (ROS) detection kit were obtained from Beyotime Biotechnology (Shanghai, China); the mitochondrial membrane potential assay (JC-1) kit, 2× SYBR Green and qPCR Master Mix (Low ROX) were purchased from Servicebio (Wuhan, China); rabbit anti-Cyt-C monoclonal antibody, rabbit anti-Bcl-2 monoclonal antibody, rabbit anti-Bax monoclonal antibody, rabbit anti-Caspase-3 monoclonal antibody and β-action antibody were obtained from Cell Signaling Technology (CST, Boston, MA, USA).
Reactive oxygen species detection kit
Manufactured by Beyotime
Sourced in China
The Reactive Oxygen Species (ROS) Detection Kit is a laboratory tool designed to detect and measure the presence of reactive oxygen species in biological samples. The kit provides a reliable and sensitive method to quantify various ROS, such as superoxide, hydrogen peroxide, and hydroxyl radicals, without interpretation or extrapolation on the intended use.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (4 mentions)
Hoechst 33342, by Beyotime (3 mentions)
Dihydroethidium kit, by Beyotime (3 mentions)
Bca protein concentration assay kit, by Beyotime (2 mentions)
Rpmi 1640 cell culture medium, by Thermo Fisher Scientific (2 mentions)
Cell cycle detection kit, by Beyotime (2 mentions)
Protease inhibitor, by Boster Bio (2 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (2 mentions)
Anti ki67, by Wuhan Servicebio Technology (2 mentions)
Tunel reaction solution, by Boster Bio (2 mentions)
Annexin 5 fitc pi apoptosis detection reagent, by BD (2 mentions)
Membrane 2 kd, by Solarbio (2 mentions)
Anti caspase 9, by Wuhan Servicebio Technology (2 mentions)
Anti caspase 3, by Wuhan Servicebio Technology (2 mentions)
Cell lysate, by Boster Bio (2 mentions)
Anti p53, by Wuhan Servicebio Technology (2 mentions)
H e staining kit, by Keygen Biotech (2 mentions)
Methyl thiazolyl tetrazolium (mtt), by Beyotime (2 mentions)
Annexin 5 fitc apoptosis kit, by Beyotime (1 mentions)
30 protocols using reactive oxygen species detection kit
1
Anticancer Effects of Siraitia Grosvenorii
2
Evaluating Apoptosis in HepG2 and Hepa1-6 Cells
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Human hepatocellular carcinoma cells, HepG2 cells (the Cell Bank of the Chinese Academy of Sciences, Beijing, China) and Hepa1-6 (the Cell Bank of the Chinese Academy of Sciences), were transfected by Shengbo Biomedical Technology Co. Ltd. (Shanghai, China). Fetal bovine serum (FBS) (Gibco, Logan, UT, USA), double resistance (Gibco), Dulbecco’s modified Eagle medium (DMEM) (Gibco), enhanced Cell Counting Kit-8 (Enhanced CCK-8 kit) (Beyotime Biotechnology, Shanghai, China), Hoechst 33258 (Beyotime Biotechnology), total SOD activity determination kit (Beyotime Biotechnology), reactive oxygen species (ROS) detection kit (Beyotime Biotechnology), hematoxylin and eosin staining reagent (Beyotime Biotechnology), P-ERK, ERK, PI3K, caspase-3, CDK4 and other antibodies (Cell Signaling Technology, Boston, MA, USA), Tranzol Up (Alltech Biotechnology Co. Ltd., Beijing, China), Prime Scrip RT reagent (Beyotime Biotechnology). ScripTM RT reagent kit with gDNA Eraser, PerfectStartTM Green qPCR SuperMix (TaKaRa, Osaka, Japan), primers (Jin Wei Zhi Biotechnology Co. Ltd., Suzhou, China) were used. All other kits were acquired from Sigma-Aldrich (St. Louis, MO, USA).
3
Probiotic Strains Enhance Caco-2 Cell Resilience
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Caco-2 cells were purchased from the Shanghai Cell Bank of Chinese Academy of Sciences. The strains, including L571 (Lactobacillus plantarum); YF-L904, YF-L903, and Mild1.0 (mixed strains of Streptococcus thermophilus and Lactobacillus Bulgarian); and ST-M5 (Streptococcus thermophilus), were obtained from Chr. Hansen (Hoersholm, Denmark). DMEM high glucose cell culture medium and fetal bovine serum (FBS) were purchased from Corning Technology Co., LTD. The iron reduction capacity test kit, ABTS free radical scavenging capacity test kit, and PBS buffer were purchased from Solarbio Technology Co., LTD. MTT was purchased from Sigma-Aldrich. The reactive oxygen species (ROS) detection kit, lipid oxidation (MDA) detection kit, total SOD activity detection kit (WST-8 method), ELISA kits of interleukin-6 (IL-6), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) were purchased from Beyotime Biotechnology Co., LTD. T-AOC, T-SOD, and MDA commercial kits were purchased from Nanjing Jiancheng Bio-engineering Insititute.
4
Anti-cancer Potential of Lentinula edodes
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Dried fruit body of L. edodes was obtained from Fangxian, Hubei Province, China (identified by Professor Jia-chun Chen of the Department of Traditional Chinese Medicine, Tongji Medical College of Huazhong University of Science and Technology). Anti-mouse β-actin antibody, horseradish peroxidase (HRP)-conjugated anti-mouse, and anti-rabbit secondary antibodies were provided by Sigma (St. Louis, MO, USA). Primary antibodies against Bcl-2, Bax, p53, cleaved caspase-3, p-p38MAPK, cyclin B1, cyclin D1, and CDK4 were obtained from Cell Signaling Technology (Danvers, MA, USA). Bicinchoninic acid (BCA) protein assay kit and reactive oxygen species (ROS) detection kit were purchased from Beyotime Biotechnology (Shanghai, China). Annexin V-FITC apoptosis detection kit was purchased from KeyGEN Biotech (Nanjing, China). All other reagents and chemicals were of analytical grade in this study.
5
Mesoporous Silica Nanoparticle Anticancer Assay
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Mesoporous silica dispersion was purchased from nanoComposix Company (USA), and Isoimperatorin from DESITE (China). Fetal bovine serum and RPMI1640 cell culture medium were extracted by Gibco (USA). Cell counting kit-8 (CCK-8) was purchased from Dojindo laboratories (Japan), and the Annexin V-FITC/PI apoptosis detection reagent was produced by BD (USA). Cell cycle detection kit, reactive oxygen species (ROS) detection kit, and DAPI were acquired from Beyotime Biotechnology (China). Rhodamine 123 (Rh123) was provided by Yeasen Biotechnology (China). Cy5 and membrane (2 kD) were purchased from Solarbio (China). Cell lysate, Protease inhibitor and TUNEL reaction solution were obtained from Boster (China). Anti-Ki-67, anti-P53, anti-Caspase-9 and anti-Caspase-3 antibodies were all purchased from Servicebio (China), while anti-Pan-Cadherin and anti-E-Cadherin antibodies were acquired from CST (USA). H&E staining kit was purchased from keygen BioTECH (Chnia), and human lymphoma cell line OCI-LY10 was acquired from the Shanghai Institute of Cell Research, Chinese Academy of Sciences.
6
Oxidative Stress Biomarker Assays
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Determination kits for malondialdehyde (MDA), glutathione (GSH) content, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX) were purchased from Nanjing Jiancheng Bioengineering Institute. The other suppliers are DMEM high glucose medium (HyClone); P/S antibiotic (HyClone); fetal bovine serum (Hangzhou Tianhang Biotechnology Co., Ltd.); trypsin EDTA (Jinuo Biomedical Technology Co., Ltd.); Cell Counting Kit-8 (CCK8), cell proliferation detection kit (Biosharp); reactive oxygen species (ROS) detection kit (Beyotime); Annexin V-FITC/PI apoptosis detection kit (Tianjin Sanjian Biotechnology Co., Ltd.); a UVA lamp purchased from Philips Lighting (China) Investment Co., Ltd. (the lamp power is 9W and the spectral wave is 364–366 nm); Dr-200bs enzyme labeling detector (Diatek); FACSCalibur flow meter (BD); Sco6we CO2 constant temperature incubator (Shell Lab); Cx-21 ordinary optical microscope (Olympus); and a Tgl-16 freezing centrifuge (Instrument of Hunan Xiangyi Laboratory).
7
Oxidative Stress Assay Protocol
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FeCl3•6H2O, ethylene glycol, sodium acetate (NaAc), H2O2 (30%), 2',7'-dichlorofluorescin diacetate (H2DCFDA) , and 3,3',5,5'-tetramethylbenzidine (TMB) were purchased from Sigma-Aldrich. Dulbecco's modified Eagle medium (DMEM) was purchased from Hyclone Co. Ltd. Fetal bovine serum (FBS) was from Gibco Co., Ltd. Penicillin-Streptomycin (100×) and gentamicin were from Beijing Solarbio Science & Technology Co., Ltd. Xylose lysine deocycholate (XLD) agar was from Qingdao-Hope Bio-Technology Co., Ltd. QIAprep spin miniprep kit was from QIAGEN Co. Ltd. Triton X-100, anti-LC3B antibody, rapamycin, bisBenzimide H33342 trihydrochloride were procured from Sigma-Aldrich Co. Ltd. Inc. Cell Counting Kit-8 (CCK-8) was from Dojindo Co., Ltd. RIPA Lysis Buffer, BCA Protein Assay kit and reactive oxygen species (ROS) detection kit were from Beyotime Biotechnology. ExpressPlus™ PAGE Gels was from GenScript Co., Ltd. Goat Anti-Rabbit IgG H&L (HRP) was from Abcam. SuperSignal West Pico Trial Kit was from Thermo Fisher Scientific Inc. Bovine serum albumin (BSA) was from Cell Signaling Technology, Inc.
8
Evaluating MTT-Based Cell Viability
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3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was obtained from Sigma Aldrich (USA). Dulbecco's modified eagle medium (DMEM), fetal bovine serum (FBS), trypsin-EDTA (0.05%), penicillin-streptomycin (P/S), PBS, calcein-AM, and enhanced chemiluminescence (ECL) western blotting detection reagent were purchased from Thermo Fisher Scientific (USA). GE11 polypeptide was purchased from GL Biochem Ltd. (Shanghai, China). Radioimmunoprecipitation assay (RIPA) lysis buffer, the primary antibodies of anti-PI3K (4292s), anti-p-PI3K (4228s), anti-Akt (9272s), anti-p-Akt (4060s), anti-Bcl-2 (3498s), and anti-Bax (5023s), were purchased from Cell Signaling Technology (USA). Bio-Rad protein assay kit and 30% acrylamide/bissolutionandpolyvinylidene difluoride (PVDF) membrane were obtained from Bio-Rad (USA). Annexin V-FITC/PI apoptosis detection kit, reactive oxygen species (ROS) detection kit, paraformaldehyde, 4,6-diamidino-2-phenylindole (DAPI), and Tubulin-Tracker were purchased from Beyotime Institute of Biotechnology (Shanghai, China).
9
Mesoporous Silica Nanoparticle Cytotoxicity
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Mesoporous silica dispersion was purchased from nanoComposix Company (USA), and Isoimperatorin from DESITE (China). Fetal bovine serum and RPMI1640 cell culture medium were extracted by Gibco (USA). Cell counting kit-8 (CCK-8) was purchased from Dojindo laboratories (Japan), and the Annexin V-FITC/PI apoptosis detection reagent was produced by BD (USA). Cell cycle detection kit, reactive oxygen species (ROS) detection kit, JC-1 detection kit and DAPI were acquired from Beyotime Biotechnology (China). Cy5 and membrane (2 kD) were purchased from Solarbio (China). Cell lysate, Protease inhibitor and TUNEL reaction solution were obtained from Boster (China). Anti-Ki-67, anti-P53, anti-Caspase-9 and anti-Caspase-3 antibodies were all purchased from Servicebio (China), while anti-Pan-Cadherin and anti-E-Cadherin antibodies were acquired from CST (USA). H&E staining kit was purchased from keygen BioTECH (Chnia), and human lymphoma cell line OCI-LY10 was acquired from the Shanghai Institute of Cell Research, Chinese Academy of Sciences.
10
Quantifying Cellular Reactive Oxygen
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The ROS content in the liver tissue or JS1 cells was measured using a DCFH-DA fluorescence probe by a reactive oxygen species detection kit (Beyotime). 10 μl DCFH-DA working solution was mixed with 190 μl liver homogenate supernatants and then incubated 30 min at 37°C in the dark. The fluorescence intensity was detected at an excitation wavelength of 500 nm and at an emission wavelength of 525 nm using a multimode reader (Varioskan Flash, Thermo Fisher Scientific). Results were from triplicate experiments.
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