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Gata3 clone l50 823

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GATA3 (clone L50-823) is an immunohistochemistry antibody used for the detection of GATA3 protein in biological samples. GATA3 is a transcription factor that plays a crucial role in the development and differentiation of various cell types. This antibody can be used in the analysis of GATA3 expression in research and diagnostic settings.

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Lab products found in correlation

Clone d33, by Agilent Technologies (1 mentions) Anti human pd 1 clone nat105, by Abcam (1 mentions) Benchmark ultra, by Roche (1 mentions) Bx40 microscope, by Olympus (1 mentions) Dako autostainer, by Agilent Technologies (1 mentions) Ck7 clone ov tl 12 30, by Agilent Technologies (1 mentions) Ck20 clone ks20, by Agilent Technologies (1 mentions) Cd56 clone 123c3, by Agilent Technologies (1 mentions) P40 clone bc 28, by Biocare Medical (1 mentions) Insm1 clone a 8, by Santa Cruz Biotechnology (1 mentions) Ttf 1 clone 8g7g3 1, by Agilent Technologies (1 mentions) S100 protein, by Roche (1 mentions) Mammaglobin clone 304 1a5, by Agilent Technologies (1 mentions) Ventana benchmark ultra, by Roche (1 mentions) Her2 clone 4b5, by Roche (1 mentions) Ttf 1 clone 8g7g3 1, by Roche (1 mentions) Ventana ultra view universal dab detection kit, by Roche (1 mentions) Estrogen receptor clone sp1, by Roche (1 mentions) Progesterone receptor clone 1e2, by Roche (1 mentions) P63 clone 4a4, by Agilent Technologies (1 mentions)

5 protocols using gata3 clone l50 823

1

Immunohistochemical Analysis of Biopsy Samples

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The biopsies were fixed in formalin and paraffin-embedded. Paraffin blocks containing sufficient material were selected for immunohistochemical (IHC) staining with the following anti-human monoclonal Abs: eosinophil peroxidase EPX-mAb (clone MM 25-82.2, kindly provided by Mayo Clinic, Scottsdale, USA), GATA-3 (clone L50-823, Cell Marque, Rocklin, USA), T-Bet (clone EPR9302 RabMab, Abcam, Cambridge, UK), and desmin to confirm muscle indemnity (clone D33, Dako, Glostrup, Denmark). The sections were stained with anti-human PD-L1 (clone SP263, Ventana, Bend USA) and anti-human PD-1 (clone NAT105, Abcam, Cambridge, UK). All markers, except for PD-L1, were determined using standardized automated protocols for LEICA BOND MAX II. PD-L1 expression was determined using the Benchmark ULTRA (Roche, Basel, Switzerland). Sections were examined by optical microscopy (Olympus BX40 microscope, DP2-BSW software), and digitalized images were analyzed using ImageJ software (NIH).
VILLOLDO G.M., POMBO M.T., ARIS M., CHEMI J., MANDÓ P., NAGARAJU S., CAMEAN J., BURIONI A., EGEA D., AMAT M., MELLADO J.L., MORDOH J., VILLARONGA A, & BARRIO M.M. (2023). A Th2-score in the tumor microenvironment as a predictive biomarker of response to Bacillus Calmette Guérin in patients with non-muscle invasive bladder carcinoma: A retrospective study. Oncology Research, 31(2), 207-220.
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2

Immunohistochemical Profiling of Tumor Samples

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Immunohistochemical staining was performed on routine sections. The following monoclonal antibodies were used for immunostaining: AE1/AE3 (clone AE1/3, 1:50 dilution; Dako, Carpinteria, CA), CK7 (clone OV-TL 12/30, prediluted; Dako), CK5/6 (D5/16B4 clone, prediluted; Dako), CK20 (clone Ks20.8, prediluted; Dako), synaptophysin (clone DAK-SYNAP, prediluted; Dako), chromogranin (clone LK2H10+PHE5, 1:200 dilution; Thermo Scientific, Waltham, MA), CD56 (clone 123C3, 1:50 dilution; Dako), TTF1 (clone SPT24, prediluted; Leica Biosystems, Buffalo Grove, IL), INSM1 (clone A-8, 1:150; Santa Cruz Biotechnology, Santa Cruz, CA), GATA3 (clone L50-823, 1:100 dilution; Cell Marque, Rocklin, CA), p63 (clone DAK-p63, prediluted; Dako), p40 (clone BC-28, 1:50 dilution; Biocare, Concord, CA), and uroplakin II (clone BC-21, 1:100 dilution; Biocare). Briefly, 4-μm-thick sections were deparaffinized in xylene and hydrated in graded alcohol. Immunostaining was performed using a DAKO autostainer (Agilent, Santa Clara, CA). Slides were incubated with the primary antibody and then with a visualization reagent (secondary goat anti-mouse immunoglobulin and horseradish peroxidase linked to a dextran polymer backbone). The slides were then rinsed with distilled water, incubated with a 3,3-diaminobenzidine substrate-chromogen solution, and subjected to Mayer hematoxylin counterstaining.
Wang G., Yuan R., Zhou C., Guo C., Villamil C., Hayes M., Eigl B.J, & Black P. (2021). Urinary Large Cell Neuroendocrine Carcinoma: A Clinicopathologic Analysis of 22 Cases. The American Journal of Surgical Pathology, 45(10), 1399-1408.
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3

Immunohistochemical Profiling of NTRK-Rearranged Thyroid Tumors

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IHC stains were performed using 4-μm-thick formalin-fixed paraffin-embedded (FFPE) sections of selected representative tissue blocks from the 11 NTRK-rearranged thyroid tumors. A Bond 3 automated stainer (Leica Microsystems, Bannockburn, IL) and compatible primary antibodies for S100 protein (polyclonal and prediluted; Ventana, Oro Valley, AZ), mammaglobin (clone 304-1A5; dilution 1:250; Dako, Carpinteria, CA), GATA3 (clone L50-823, dilution 1:200; Cell Marque, Rocklin, CA), PAX8(polyclonal, dilution 1:1000; Proteintech, Rosemont, IL), TTF1 (clone 8G7G3/1; dilution 1:300; Dako, Carpinteria, CA), BRAF V600E (clone VE1; dilution 1:200; Abcam, Cambridge, United Kingdom), thyroglobulin (dilution 1:800; Cell Marque, Rocklin, CA) and HBME1 (prediluted; Ventana, Oro Valley, AZ) were used. Appropriate positive and negative controls were included. Stained slides were reviewed by three pathologists (VN, PMS, and YHC) and graded as negative (0% tumor cells stained), focal (<50%) and diffuse (>50%).
Chu Y.H., Dias-Santagata D., Farahani A.A., Boyraz B., Faquin W.C., Nosé V, & Sadow P.M. (2020). Clinicopathologic and molecular characterization of NTRK-rearranged thyroid carcinoma (NRTC). Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 33(11), 2186-2197.
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4

Comprehensive IHC Staining Protocol

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Various IHC stains of relevance were performed in the clinical setting at the Dept. of Pathology, Lund, Region Skåne, Sweden, as part of the histopathological diagnostic procedure. Four micrometer thick sections from FFPE tissue blocks were pre-treated and stained in a Ventana Bench-Mark Ultra using Ventana ultraView Universal DAB Detection Kit (Ventana Medical Systems, Tucson, AZ). Recommended control tissue was used on each slide. The clones and vendors for the antibodies included in this study were TTF-1 clone 8G7G3/1, CK7 clone OV-TL 12/30, CK20 clone SP33, CDX2 clone EPR2764Y, S100 polyclonal, estrogen receptor clone SP1, progesterone receptor clone 1E2, HER2 clone 4B5, ALK clone D5F3, all Ventana Medical Systems (Tucson, AZ), napsin A clone IP64, CK5 clone XM26, both Novocastra/Leica Biosystems (Kista, Sweden), p63 clone 4A4, smooth muscle specific actin clone 1A4, both Dako (Glostrup, Denmark), p40 clone BC28, Histolab Products/Biocare Medical (Gothenburg, Sweden), and GATA3 clone L50-823, Cell Marque (Rocklin, CA).
Ericson-Lindquist K., Johansson A., Levéen P., Elmberger G., Jönsson G., Staaf J, & Brunnström H. (2017). Targeted sequencing may facilitate differential diagnostics of pulmonary tumours: a case series. Diagnostic Pathology, 12, 31.
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5

Immunohistochemical Profiling of Tumor Markers

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If not previously performed, primary monoclonal antibodies to CAM5.2 (clone CAM5.2, dilution 1:100; BD Biosciences, San Jose, CA), CD10 (clone 56D6, dilution 1:100; Leica Biosystems, Buffalo Grove, IL), GATA3 (clone L50-823, dilution 1:200; Cell Marque, Rocklin, CA), PAX8 (polyclonal, dilution 1:1000; Proteintech Group, Rosemont, IL), SF-1 (clone N1665, dilution 1:200; Thermo Fisher Scientific, Waltham, MA), and vimentin (clone V9, dilution 1:2000; DAKO, Santa Clara, CA) were applied to a representative 5-μm thick section of formalin-fixed, paraffinembedded tumor. Appropriate controls were run in tandem. Antibodies were considered positive if nuclear (GATA3, PAX8, SF-1), cytoplasmic (CAM5.2, vimentin), or cytoplasmic/membranous (CD10) staining was present, and interpreted as diffusely positive (>50% staining), focally positive (<50% staining), or negative.
Bennett J.A., Ritterhouse L.L., Furtado L.V., Lastra R.R., Pesci A., Newell J.M., Burandt E., Kooreman L., Van de Vijver K., Krausz T., Felix A., Zannoni G.F., Young R.H, & Oliva E. (2020). Female adnexal tumors of probable Wolffian origin: morphological, immunohistochemical, and molecular analysis of 15 cases. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 33(4).
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