Dox s1208

Two 3D-HCC cancer in vitro models (CSM and ASM) were used in drug sensitivity assays to identify changes in drug sensitivity for each model. The drugs most commonly used for HCC were selected. Sorafenib (S7397), Cabozantinib (S1119), Lenvatinib (S1164), Regorafenib (S1178), 5-FU (S1209), and DOX (S1208) were purchased from Selleckchem (Houston, TX, USA). Drugs were dissolved in a stock solution of dimethyl sulfoxide (DMSO, 100 mM).

DOX (S1208) was purchased from Selleck, and oseltamivir (HY-17016) was obtained from MedChemExpress (MCE). ELISA kits for NEU1 and N-acetylneuraminic acid (Neu5Ac) were purchased from Jingmei Biotechnology (Jiangsu, China). ELISA kits for creatine kinase isoenzyme-MB (CK-MB) and cardiac troponin T (cTnT) were obtained from Meilian Biotechnology. Commercial assay kits of aspartate aminotransferase (AST), lactate dehydrogenase (LDH), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), reduced glutathione (GSH) activities, and hydrogen peroxide (H₂O₂) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay was obtained from Beyotime Biotechnology (Shanghai, China). Cell Mitochondria Isolation Kit was purchased from Beyotime Biotechnology (China). All primary antibodies used in this study are listed in Supplementary Table S1.