Anti cd45 af700

Manufactured by Thermo Fisher Scientific

Anti-CD45-AF700 is a fluorescently labeled monoclonal antibody that binds to the CD45 protein, a common leukocyte antigen expressed on the surface of most hematopoietic cells. This product is intended for use in flow cytometry applications to identify and characterize cell populations.

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Anti-CD45 (119 citations) CD45-AF700 (6 citations)

3 protocols using anti cd45 af700

Multiparametric Flow Cytometry of Murine Lungs

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On day 7, lungs were collected aseptically after mice were euthanized. Lungs were passed through 70 um cell strainer to obtain single-cell homogenates. Red blood cells were lysed and the remaining cells were washed with FACS buffer. After cells were resuspended in 200 ul of FACS buffer, Fc receptors were blocked with anti-CD16/CD32 (Fc Block, BD) and then stained using the following antibodies (all from eBiosciences): anti-CD11b-APC, anti-CD11c-PECy7, anti-CD45-AF700, anti-Gr1-PerCpCy5.5, viability-e520, anti-Ly6C-PE, anti-MHCII-e450, anti-SiglecF-PE-CF594.

Choi A., Ibañez L.I., Strohmeier S., Krammer F., García-Sastre A, & Schotsaert M. (2020). Non-sterilizing, Infection-Permissive Vaccination With Inactivated Influenza Virus Vaccine Reshapes Subsequent Virus Infection-Induced Protective Heterosubtypic Immunity From Cellular to Humoral Cross-Reactive Immune Responses. Frontiers in Immunology, 11, 1166.

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Multi-parameter Flow Cytometry for Immune Profiling

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For flow cytometry analysis anti-CD45 AF700, anti-CD45.2 APC-eF780, anti-CD45.1 eF480, anti-CD90.2 APC, anti-CD69 FITC, anti-CD90.1 PE-Cy7, anti-CCR7 PE (eBioscience), anti-CD4 PE TexasRed (Invitrogen), anti-CD8b PerCp/Cy5.5, and anti–IFN-γ PE-Cy7 (BioLegend) were used. For in vivo CD4 depletion mice received i.p. injections of 250 µg GK1.5 on days −5 and −2 before challenge. For in vivo CXCR3 blockade mice received i.p. injections of 250 µg anit-CXCR3-173 mAb (BioXcell) one day before challenge. For intravascular staining mice were injected with 3 µg FITC anti-CD45.2 (eBioscience) 3 min before they were euthanized by CO₂. The data from flow cytometry were collected using LSRII flow cytometer (BD) and analyzed using FlowJo software (Tree Star).

Glennie N.D., Yeramilli V.A., Beiting D.P., Volk S.W., Weaver C.T, & Scott P. (2015). Skin-resident memory CD4+ T cells enhance protection against Leishmania major infection. The Journal of Experimental Medicine, 212(9), 1405-1414.

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Antibody Staining Protocol for Immune Cell Analysis

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Antibodies to GSDMD (ab219800 and ab209845) and MUC2 (AB76774) were from Abcam. Anti–α-tubulin (T9026) and anti–β-actin (A1978) were from Sigma-Aldrich. Anti–caspase-11 (NB120-10454) was supplied from Novus Biologicals. Anti-ASC (sc-22514) was from Santa Cruz Biotechnology. Anti-phosphorylated STING (72971s), anti-STING (13647s), anti-phosphorylated IRF3 (4947s), anti-IRF3 (4302s), anti-TBK1 (3013s), and anti-phosphorylated TBK1 (5483s) were from Cell Signaling Technology. DSS (DB001-38) was from TdB Consultancy. PAS staining kit was from Servicebio (G1008). Alcian blue staining kit was from Solarbio (G1560). Anti–CD45–fluorescein isothiocyanate (FITC) (30-F11,11-0451-82), anti–CD8a–phycoerythrin (PE) (53-6.7,12-0081-83), anti–CD45-AF700 (30-F11,85-11-0112-81), anti–CD11b-FITC (M1/70,85-12-0114-81), anti-F4/80 (BM8,17-4801-82), anti–Ly6c-PE-Cy7 (HK1.4,25-5932-82), anti–Ly6g–eFlour 450 (1A8-LY6G,48-9668-82), and FVD (fixable viability dyes)–eFlour 506 (65-0866) were from eBioscience. Anti–CD4-APC (allophycocyanin)–Cy7 (GK1.5,100414) was from BioLegend. Anti–CD11b-PE (M1/70, 557397) was from BD Biosciences.

Ma C., Yang D., Wang B., Wu C., Wu Y., Li S., Liu X., Lassen K., Dai L, & Yang S. (2020). Gasdermin D in macrophages restrains colitis by controlling cGAS-mediated inflammation. Science Advances, 6(21), eaaz6717.

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