The microglial cells were collected and washed twice with PBS. The single cell suspensions were blocked by 0.1% Triton X-100 and 3% BSA in PBS, and then stained with CD86-FITC (rat) and CD206-PE (rat) antibodies (BD Biosciences, La Jolla, CA, USA) by the manufacturer's protocols, respectively. The microglial cells were examined with a BD FACSVerse flow cytometer (BD Biosciences), and all of the tests were controlled by the homologous isotype control antibodies.
Cd206 pe rat antibodies
Manufactured by BD
Sourced in United States
CD206-PE (rat) antibodies are a type of laboratory reagent used for the detection and analysis of CD206-expressing cells in research applications. CD206 is a receptor that is expressed on the surface of certain types of immune cells, such as macrophages. The CD206-PE (rat) antibodies are conjugated with the fluorescent dye phycoerythrin (PE), which allows for the visualization and quantification of CD206-positive cells using flow cytometry or other fluorescence-based techniques.
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Lab products found in correlation
2 protocols using cd206 pe rat antibodies
1
Microglial Phenotypic Characterization
2
Phenotyping Microglia Activation States
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The microglia were collected and washed twice with PBS, and blocked by 0.1% Triton X‐100 and 3% BSA in PBS. The microglia were then stained with CD86‐FITC (rat) and CD206‐PE (rat) antibodies (BD Biosciences, La Jolla, CA, USA), respectively. The microglia were examined with a BD FACSVerse flow cytometer (BD Biosciences), and all of the tests were controlled by the homologous isotype control antibodies.
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