Log In Sign up
The largest database of trusted experimental protocols

Rhodamine red x goat anti rabbit igg

Manufactured by Thermo Fisher Scientific
Visit Supplier

Rhodamine Red-X goat anti-rabbit IgG is a fluorescently-labeled secondary antibody. It is designed to bind to rabbit immunoglobulin G (IgG) for detection purposes in various immunoassays and microscopy techniques.

Automatically generated - may contain errors

Lab products found in correlation

Diva decloaker, by Biocare Medical (2 mentions) Rm 2135rotary microtome, by Thermo Fisher Scientific (2 mentions) Primaryantibody galns, by Abcam (2 mentions) Collagenx, by Santa Cruz Biotechnology (2 mentions) Prolong gold with dapi, by Thermo Fisher Scientific (2 mentions) Superfrost plus slide, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488, by Jackson ImmunoResearch (2 mentions) Rabbit anti 5 ht, by Merck Group (1 mentions) Mouse anti calbindin d 28k, by Merck Group (1 mentions) Alexa fluor 488 goat anti chicken igg, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)

Close spelling variants of this product

Rhodamine Red-X IgG Rabbit

4 protocols using rhodamine red x goat anti rabbit igg

1

Embryonic and Adult Brain Immunostaining

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pregnant females were sacrificed by cervical dislocation, the embryos dissected out of the uterus and fixed o/n in 4% paraformaldeyde (PFA) at 4°C. Adult animals were anaesthetized with Avertin and perfused intracardially with 4% PFA. Brains were dissected out, post-fixed o/n in PFA at 4°C and embedded in either 2.5% agarose or tissue-tek for sectioning with vibratome or cryostat, respectively.
For immunostaining specimens were incubated with primary antibodies o/n at 4°C in PBS containing 5% heat-inactivated lamb serum and 0.5% Triton X-100. Primary antibody dilutions: rabbit anti-5-HT (Sigma) 1∶500; chicken anti-eGFP/eYFP (Abcam) 1∶1000; mouse anti-calbindin-D-28K (Sigma), 1∶200. Fluorescent-conjugated secondary antibody were used as follow: Rhodamine Red-X goat anti-rabbit IgG 1∶500; Alexa Fluor 488 goat anti-chicken IgG 1∶200; Rhodamine Red-X goat anti-mouse IgG 1∶500 (all by Molecular Probes). Cell nuclei were counterstained with DAPI (Sigma), 0.5 µg/ml.
Pelosi B., Migliarini S., Pacini G., Pratelli M, & Pasqualetti M. (2014). Generation of Pet1210-Cre Transgenic Mouse Line Reveals Non-Serotonergic Expression Domains of Pet1 Both in CNS and Periphery. PLoS ONE, 9(8), e104318.
+ Open protocol
+ Expand
2

Immunohistochemistry of Testicular Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Freshly dissected testes were fixed in 4% paraformaldehyde, embedded in paraffin and sectioned at 5 μm. The sections were deparaffinized in xylene and an ethanol series. Antigen retrieval was performed by boiling the sections for 20 min in 10 mM tribasic sodium citrate, pH 6. The samples were washed in phosphate-buffered saline (PBS) and then immersed in 0.4% Triton X-100 for 10 min before being blocked with a buffer (3% bovine serum albumin in PBS) for 30 min. The sections were incubated overnight at 4°C with the primary antibodies (5 μg·mL-1), and then placed in 1/1000 dilutions of Rhodamine Red™-X goat anti-rabbit IgG (Molecular Probes) for 30 min at room temperature. DNA was stained with DAPI, and the sections were mounted and visualized by fluorescence microscopy (DMLB, Leica).
Kwon J.T., Ham S., Jeon S., Kim Y., Oh S, & Cho C. (2017). Expression of uncharacterized male germ cell-specific genes and discovery of novel sperm-tail proteins in mice. PLoS ONE, 12(7), e0182038.
+ Open protocol
+ Expand
3

Immunohistochemistry of GALNS and Collagen X

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissues were processed into paraffin, sections cut on a Leica RM 2135
rotary microtome and dried overnight at 36°C on Fisher Superfrost Plus
slides. Sections were deparaffinized and rehydrated, and antigen retrieval
performed (120°C, 3 minutes) using DIVA Decloaker (Biocare Medical).
After washing in distilled water twice for 3 minutes, sections were blocked in
PBS containing 5% normal goat serum, 1% bovine serum albumin, and 0.25% triton
X-100 in a humidified chamber for 1 hour at RT, then incubated in primary
antibody GALNS (Epitomics) 1:50, normal rabbit IgG in block solution or Collagen
X (Santa Cruz Biotechnology) 1:100 for 2 hours at RT. After 3 washes for 5
minutes in PBS, sections were incubated in goat anti-rabbit IgG Rhodamine Red-X
(Invitrogen) 1:300 or Alexa Fluor 488 (Jackson Immunoresearch) in block solution
for 1 hour at RT in the dark, washed 3 times for 5 minutes in PBS in the dark,
rinsed in distilled water, and coverslipped in Prolong Gold with DAPI
(Invitrogen).
Alméciga-Díaz C.J., Montaño A.M., Barrera L.A, & Tomatsu S. (2018). Tailoring the AAV2 capsid vector for bone-targeting. Pediatric research, 84(4), 545-551.
+ Open protocol
+ Expand
4

Immunohistochemistry of GALNS and Collagen X

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissues were processed into paraffin, sections cut on a Leica RM 2135
rotary microtome and dried overnight at 36°C on Fisher Superfrost Plus
slides. Sections were deparaffinized and rehydrated, and antigen retrieval
performed (120°C, 3 minutes) using DIVA Decloaker (Biocare Medical).
After washing in distilled water twice for 3 minutes, sections were blocked in
PBS containing 5% normal goat serum, 1% bovine serum albumin, and 0.25% triton
X-100 in a humidified chamber for 1 hour at RT, then incubated in primary
antibody GALNS (Epitomics) 1:50, normal rabbit IgG in block solution or Collagen
X (Santa Cruz Biotechnology) 1:100 for 2 hours at RT. After 3 washes for 5
minutes in PBS, sections were incubated in goat anti-rabbit IgG Rhodamine Red-X
(Invitrogen) 1:300 or Alexa Fluor 488 (Jackson Immunoresearch) in block solution
for 1 hour at RT in the dark, washed 3 times for 5 minutes in PBS in the dark,
rinsed in distilled water, and coverslipped in Prolong Gold with DAPI
(Invitrogen).
Alméciga-Díaz C.J., Montaño A.M., Barrera L.A, & Tomatsu S. (2018). Tailoring the AAV2 capsid vector for bone-targeting. Pediatric research, 84(4), 545-551.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!