Nanogel samples have been labelled with N-succinimidyl [3H]-propionate (Moravek, Brea, CA) as previously described (Vinogradov et al. 2004a (link)). Each 3H-NG/CTP formulation was injected in the tail vein of C57BL/6 mice (3 animals per group), and its amount in blood and organs (brain, liver, spleen, kidney, lung and heart) was measured at different doses and time points. Organs were analyzed by dissolving in 1 mL Solvable solution (Perkin Elmer, Santa Clara, CA) per 100mg tissue for 1h at 60°C. Samples were treated with 0.1 mL of 0.2M EDTA and 0.2 mL of hydrogen peroxide (30% v/v) overnight, and then mixed with 0.1 mL of 1N HCl HCl. Half of the tissue solution was added to 2mL of Ultima Gold Scintillation Mixture (Perkin Elmer) in glass scintillation vial, and the sample radioactivity was measured using a 2500TR/RB Packard Tricarb liquid scintillation analyzer (Perkin Elmer).
Ultima gold scintillation mixture
Manufactured by PerkinElmer
Sourced in United States
Ultima Gold Scintillation Mixture is a high-performance liquid scintillation cocktail designed for use in liquid scintillation counting. It is an efficient and versatile solution that provides stable and reproducible results for a wide range of sample types and counting applications.
Automatically generated - may contain errors
Lab products found in correlation
Solvable solution, by PerkinElmer (1 mentions)
Pk11195, by Merck Group (1 mentions)
Protease inhibitor, by Merck Group (1 mentions)
Penicillin, by Lonza (1 mentions)
Streptomycin, by Lonza (1 mentions)
L glutamine, by Lonza (1 mentions)
Protein assay reagent, by Bio-Rad (1 mentions)
Fetal bovine serum (fbs), by Lonza (1 mentions)
Dulbecco s modified eagle s medium, by Lonza (1 mentions)
4 phenylbutyric acid, by Merck Group (1 mentions)
3h mpp, by PerkinElmer (1 mentions)
Cell culture media, by Merck Group (1 mentions)
3 protocols using ultima gold scintillation mixture
1
Biodistribution of Radiolabeled Nanogels
2
Radioligand Binding Assay Protocol
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[3H]PK11195 (Specific Activity, 85.7 μCi/nmol) and Ultima Gold scintillation mixture were obtained from Perkin-Elmer Life Sciences. PK11195, protease inhibitors and GF/C glass fiber filters were purchased from Sigma-Aldrich. 2-arylindol-3-ylglyoxyl derivatives were synthesized as previously described27 (link)28 (link)29 (link). Dulbecco’s modified Eagle’s medium, fetal bovine serum, L-glutamine, penicillin, and streptomycin were from Lonza (Milano, Italy). Enzyme immunoassay (ELISA) for pregnenolone measurement was obtained from IBL (Hamburg, Germany). SU10603 and trilostane were gifts from Novartis Farma (Varese, Italy) and Dr. Zister (University of Dublin, Dublin, Ireland), respectively. Protein assay reagent was obtained by Bio-Rad Laboratories Inc. All other chemical reagents were obtained by commercial sources.
3
Radiolabeled MPP+ Uptake Assay
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[3H]MPP+ (N-methyl-4-phenylpyridinium, 82.9 Ci/mmol) and ULTIMA Gold scintillation mixture were purchased from PerkinElmer Life Science (Waltham, MA, USA). Decynium-22 was ordered from Synthon Chemicals (Bitterfeld, Wolfen, Germany). All other chemicals, including 4-phenyl butyric acid, and cell culture media were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Sarstedt (Nuembrecht, Germany).
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