Annexin 5 fitc apoptosis detection kit

As previously described,^{[15 (link)]} flow cytometry (FCM) was used to analyze cell cycle and apoptosis. The cell cycle was analyzed by ethanol-fixed cells stained with propidium iodide (PI) in buffer containing RNase A (Cat: FXP0211, 4A Biotech Co., Ltd, Beijing, China). The DNA content was assessed with a FACS Calibur flow cytometer (BD Biosciences, USA). Cell apoptosis was detected using the Annexin V-FITC Apoptosis Detection Kit (Cat: FXP022, 4A Biotech Co., Ltd, Beijing, China). The percentage of apoptotic cells were calculated with CellQuest 6.0 (BD Biosciences, USA).

An Annexin‐V‐FITC apoptosis detection kit purchased from 4A Biotech Co., Ltd. was applied to detect cell apoptosis. Cells were exposed to RA or DDP alone or in combination for 48 hr, harvested in cold phosphate‐buffered saline, resuspended in 500 μl incubation buffer containing Annexin‐V‐FITC and PI, incubated in the dark for 30 min, and analyzed with the ACEC NovoCyte flow cytometer equipped with Novoexpress.

The Annexin V‐FITC apoptosis detection kit purchased from 4A Biotech Co., Ltd. was applied to detect cell apoptosis. A549 and PC9 cells (5.0 × 10⁵/1 ml/well) in logarithmic phase were plated in six‐well plates and treated with different drugs for 48 hr. The treated cells were collected and washed with cold PBS after treatment. In accordance with the manufacturer's instructions, the cells were stained with Annexin V‐FITC and propidium iodide for 30 min at room temperature in the dark, and the apoptosis rate of the treated cells was determined immediately after staining by using an ACEC NovoCyte flow cytometer equipped with Novoexpress (Becton Dickinson, San Jose, CA, USA).