Coomassie brilliant blue r 250 cbb

Manufactured by Merck Group

Sourced in United States

Coomassie Brilliant Blue R-250 (CBB) is a laboratory dye used for the detection and quantification of proteins. It is a blue, water-soluble dye that binds to proteins, forming a colored complex that can be measured spectrophotometrically. CBB is commonly used in protein assays, such as the Bradford assay, to determine the concentration of proteins in a sample.

Automatically generated - may contain errors

Lab products found in correlation

5 protocols using coomassie brilliant blue r 250 cbb

Analyzing Porcine Chia and Pepsin Proteins

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

The obtained protein fractions were analyzed using standard SDS-PAGE, followed by Coomassie Brilliant Blue R-250 (CBB, Sigma-Aldrich) or WB using anti-porcine N-terminal Chia (rabbit)^{12 (link)} anti-mouse C-terminal Chia (rabbit)^{44 (link)} or anti-porcine pepsin antibody (donkey) (GeneTex, Irvine, CA, USA), followed by peroxidase-conjugated AffiniPure F (ab’)₂ Fragment Donkey Anti-Rabbit IgG (H + L) (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) or AffiniPure Donkey Anti-Goat IgG-HRP (Jackson ImmunoResearch laboratories). The immunoblots were analyzed by Luminescent Image Analyzer (ImageQuant LAS 4000, GE Healthcare, Piscataway, NJ, USA) according to the manufacturer’s instructions.

Tabata E., Wakita S., Kashimura A., Sugahara Y., Matoska V., Bauer P.O, & Oyama F. (2019). Residues of acidic chitinase cause chitinolytic activity degrading chitosan in porcine pepsin preparations. Scientific Reports, 9, 15609.

+ Open protocol

+ Expand

SARS-CoV-2 S1-RBD Protein ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lysozyme (Lys), bovine serum albumin (BSA), dinitrophenyl hydrazine (DNPH), glycine, tris (hydroxymethyl) aminomethane, Tween-20, ammonium persulphate, sodium dodecyl sulphate (SDS), para-nitrophenyl phosphate, goat anti-human IgG alkaline phosphatase conjugate (ALP), and Coomassie brilliant blue-R250 (CBB) were purchased from Sigma-Aldrich, St. Louis, Missouri, USA. Recombinant S1-RBD-protein and anti-R-C19-S1-RBD IgG (MyBioSource, San Diego, CA, USA). Enzyme-linked immunosorbent assay (ELISA) polystyrene microtiter flat-bottom (96-well) plates were sourced from Nunc (Sigma-Aldrich, St. Lois, Missouri, USA). All chemicals were of analytical grade.

Sherwani S., Raafat M., Rajendrasozhan S., Khan M., Saleem M., Husain Q., Khan S., Alam N, & Khan M.W. (2022). Increased Levels of Autoantibodies against ROS-Modified Proteins in Depressed Individuals with Decrease in Antibodies against SARS-CoV-2 Antigen (S1-RBD). Current Issues in Molecular Biology, 44(11), 5260-5276.

+ Open protocol

+ Expand

Protein Fractionation and Immunoblotting Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The obtained protein fractions were analyzed using standard SDS-PAGE, followed by Coomassie Brilliant Blue R-250 (CBB, Sigma-Aldrich, St. Louis, MO, USA) or Western blot using polyclonal anti-mouse C-terminal AMCase^{33 (link)} or polyclonal pig anti-pepsin antibody (GeneTex, Irvine, CA, USA), followed by peroxidase-conjugated AffiniPure F (ab’)₂ Fragment Donkey Anti-Rabbit IgG (H + L) (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) or AffiniPure Donkey Anti-Goat IgG-HRP (Jackson ImmunoResearch laboratories). The immunoblots were analyzed and quantified by Luminescent Image Analyzer (ImageQuant LAS 4000, GE Healthcare, Piscataway, NJ, USA) according to the manufacturer’s instructions.

Tabata E., Kashimura A., Wakita S., Ohno M., Sakaguchi M., Sugahara Y., Imamura Y., Seki S., Ueda H., Matoska V., Bauer P.O, & Oyama F. (2017). Protease resistance of porcine acidic mammalian chitinase under gastrointestinal conditions implies that chitin-containing organisms can be sustainable dietary resources. Scientific Reports, 7, 12963.

+ Open protocol

+ Expand

Characterization of Digestive Enzymes

Check if the same lab product or an alternative is used in the 5 most similar protocols

α-Amylase from human saliva (EC 3.2.1.1; A0521-500 UN; Type IX-A, lyophilized powder 1000–3000 U·mg⁻¹ protein) and porcine pepsin from gastric mucosa (EC 3.4.23.1; P6887-1G, lyophilized powder, 3200–4500 U·mg⁻¹ protein) were purchased from Sigma–Aldrich (Saint-Louis, MO, USA). The enzyme activities were measured according to the assays detailed by Minekus et al., (2014) [15 (link)]. Chemicals for gel electrophoresis (Tris, glycine, CHAPS, urea, thiourea, dithiothreitol (DTT), acrylamide, bis-acrylamide, trichloroacetic acid (TCA), and Coomassie brilliant blue R-250 (CBB)) were purchased from Sigma-Aldrich. Ampholytes and immobilized pH gradient strips (IPG) were supplied by GE Healthcare (Uppsala, Sweden). All chemicals were analytical reagent grade, and mili-Q water (18 MΩ cm at 25 °C) (Millipore, Bedford, MA, USA) was used for all experiments.

Prodić I., Smiljanić K., Nagl C., Ballmer-Weber B., Hoffmann-Sommergruber K, & Veličković T.Ć. (2022). INFOGEST Digestion Assay of Raw and Roasted Hazelnuts and Its Impact on Allergens and Their IgE Binding Activity. Foods, 11(18), 2914.

+ Open protocol

+ Expand

Recombinant Protein Expression in E. coli

Check if the same lab product or an alternative is used in the 5 most similar protocols

E. coli host strains BL21(DE3), were obtained from Novagen (Wisconsin, USA). Synthesis of construct (based on sequence from protein data base, UniProt ID ID P0DTC9) cloned in NdeI and XhoI sites of plasmid pET20b, was ordered from Genscript (Piscataway, New Jersey, United States). Agar, tryptone and yeast extract were purchased from Torlak (Institute for virology, vaccines and serums, Belgrade, Serbia). Imidazole, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and nitro blue tetrazolium (NBT) were purchased from Carl Roth, (Germany) isopropyl-d-thiogalactopyranoside (IPTG), ampicillin-sodium salt and Tris – base were purchased from Fisher Scientific (Loughborough, UK). Immobilized metal ion affinity chromatography (IMAC) resin, Ni Sepharose 6 fast Flow, and SP Sepharose were purchased from GE Healthcare (Uppsala, Sweden). 6x-His Tag Mouse anti-Tag, alkaline phosphatase (AP) monoclonal antibodies were purchased from Invitrogen (Carlsbad, California, United States). Coomassie Brilliant Blue R-250 (CBB) from Sigma (St Louis, MO, USA).

Djukic T., Mladenovic M., Stanic-Vucinic D., Radosavljevic J., Smiljanic K., Sabljic L., Devic M., Cujic D., Vasovic T., Simovic A., Radomirovic M, & Cirkovic Velickovic T. (2021). Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2. Virology, 557, 15-22.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!