Uapon tirf 100

Experiments were performed in two MT-TIRF setups similar to one described before (Madariaga-Marcos et al., 2018 (link)). In brief, 488 nm laser source (Vortran Stradus) was focused on the back focal plane of a high numerical aperture objective (Olympus UAPON TIRF 100×). We used two separate detectors to visualise the emission of the fluorophores in the sample and the magnetic beads; an EM-CCD temperature-controlled camera (Andor Ixon Ultra 897/Andor Ixon Ultra 888) and a CCD or CMOS camera (Pulnix 6710 CL/Mikrotron MC1362) for bright-field video microscopy. Lateral Magnetic Tweezers consisted of a pair of permanent magnets (Q-05-05-02-G, Supermagnete) connected to a linear motor (Piezomotor). The MT setup was controlled by a custom-written code in Umbarger et al. (2011) (link). Fluorescence camera was controlled by Andor Solis software.

A 488 nm laser source (Vortran Stradus) was focused on the back focal plane of a high numerical aperture objective (Olympus UAPON TIRF 100×). We used two separate detectors to visualize the emission of the fluorophores in the sample and the magnetic beads; an EM-CCD temperature-controlled camera (Andor Ixon Ultra 897) and a CCD camera (Pulnix 6710CL) for bright-field video microscopy. The fluorescence and bright-field signals were separated using a dichroic mirror, which permits using a single optical path for both detectors (Fig. S11†).

Experiments were performed in a MT-TIRF setup similar to a one described before^{33 (link)}. In brief, a 488 nm laser source (Vortran Stradus) was focused on the back focal plane of a high numerical aperture objective (Olympus UAPON TIRF 100 ×). Two separate detectors were used to visualize the emission of the fluorophores in the sample and the magnetic beads; an EM-CCD temperature-controlled camera (Andor Ixon Ultra 888) and a CCD or CMOS camera (Pulnix 6710CL/Mikrotron MC1362) for bright-field video microscopy. The MT setup was controlled by a custom-written code in LabVIEW 2011. The fluorescence camera was controlled by the Andor Solis software (Solis version 4.30.30036.0; SDK version 2.102.33036.0; www.andor.com).