Zebrafish larvae were imaged in an imaging system that holds four 96-well plates for automated analysis of behavior in a 384-well format as previously described [10 (link)]. Briefly, the imaging system is housed in a 28.5ºC temperature-controlled cabinet where larvae in white 96-well ProxiPlates are placed onto a glass stage. Above the stage, a high-resolution camera (18-megapixel Canon EOS Rebel T6 with an EF-S 55–250 mm f/4.0–5.6 IS zoom lens) captures an image of the larvae in the four 96-well plates every 6 seconds. The camera is connected to a continuous power supply (Canon ACK-E10 AC Adapter) and controlled by a laptop computer using Canon’s Remote Capture software (EOS Utility, version 3), which is included with the camera. Unlike previous descriptions of this imaging system, two small speakers (OfficeTec USB Computer Speakers Compact 2.0 System) were attached speaker-side down to the glass stage. Speakers were connected by USB to the laptop computer and set to maximum volume, reaching 85 dBA on the stage. Below the glass stage, a M5 LED pico projector (Aaxa Technologies) with a 900 lumens LED light source displays Microsoft PowerPoint presentations through the opaque bottom of the 96-well plates.
Remote capture software eos utility version 3
Manufactured by Canon
EOS Utility (version 3) is a software application developed by Canon for remote capture and control of Canon EOS digital cameras. The software enables users to control camera settings, capture images, and transfer images from the camera to a computer.
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Lab products found in correlation
2 protocols using remote capture software eos utility version 3
1
Automated Behavioral Analysis of Zebrafish Larvae
2
Automated Zebrafish Behavioral Imaging
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Zebrafish larvae were imaged in an imaging system that holds four 96-well plates for automated analysis of behavior in a 384-well format (Fig. 1 ). The plates were imaged as described previously19 (link). Briefly, the imaging system is housed in a 28.5ºC temperature-controlled cabinet where larvae in white 96-well ProxiPlates are placed onto a glass stage. Above the stage, a high-resolution camera (18-megapixel Canon EOS Rebel T6 with an EF-S 55–250 mm f/4.0–5.6 IS zoom lens) captures an image of the larvae in the four 96-well plates every 6 s. The camera is connected to a continuous power supply (Canon ACK-E10 AC Adapter) and controlled by a laptop computer using Canon’s Remote Capture software (EOS Utility, version 3), which is included with the camera. Two small speakers (OfficeTec USB Computer Speakers Compact 2.0 System) were attached speaker-side down to the glass stage. Speakers were connected by USB to the laptop computer and set to maximum volume (85 dBA). A M5 LED pico projector (Aaxa Technologies) with a 900 lumens LED light source is located below the glass stage. This projector is used for background illumination and the display of visual stimuli, using the opaque bottom of the 96-well plates as a rear projection screen.
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