Guanidine chloride solution

Reactions contained 1 X WarmStart^® Colorimetric LAMP Master Mix (M1800; NEB) supplemented with 1 x EvaGreen (Biotium), 0.02 U/μL Antarctic Thermolabile UDG (NEB), 700 μM dUTP (NEB), 1 X standard concentration LAMP primers and 40 mM guanidine chloride solution (Sigma G3272, pH adjusted to pH ~8). Reactions were prepared to final volume of 25 μl using nuclease free water, incubated at 65°C for 40 minutes on a StepOnePlus thermocycler (Applied Biosystems). The colour of finished reactions was recorded using an office flatbed scanner.

Aluminium chloride (99%), ammonium bicarbonate (≥99.5%), L-aspartic acid disodium salt (≥98%), L-alanine (≥99.5), β-alanine (≥99%), calcium chloride dihydrate (≥99%), chloroform (100–200 ppm amylene stabiliser, ≥99.5), cobalt chloride hexahydrate (cell culture grade), glycine (≥99%), glutamate (≥99%), guanidine chloride solution (8 M), hydrochloric acid (≥37%), isobutyl chloroformate (98%), manganese sulfate (cell culture grade), oxaloacetic acid (≥97%), potassium chloride (99%), potassium phosphate monobasic (99.5–101.0%), pyridine (98%), pyridoxamine dihydrochloride (cell culture grade), pyridoxal hydrochloride (≥99%), L-serine (≥99%), sodium acetate (99–101%), sodium orthovanadate (99.98%), and zinc chloride x (≥98%) were purchased from Sigma. Boric acid (analytical grade), water (HPLC grade) and acetonitrile (HPLC grade) were purchased from Fischer. Copper sulfate hexahydrate (98.0–102.0%), isobutanol (99%), magnesium chloride hexahydrate (≥98%), and nickel chloride hexahydrate (98%) were from Alfa Aesar. Sodium hydroxide was purchased from VWR, and sodium chloride (research-grade) was purchased from Millipore. Ammonium chloride (analysis-grade) was from Acros and 9-fluroenylmethoxycarbonyl chloride (97%) was from Novabiochem. Helium (99.995%) and Nitrogen (99.998%) were purchased from BOC.