The quantification of acetaminophen in tablets was performed using Ultimate 3000 HPLC (USA) on a C18 (150 x 4.6 mm, 5 µm, Phenomenex, USA) column. The mobile phase containing buffer solution (pH 3.5, adjusted by phosphoric acid) and acetonitrile at the ratio 3:1 was controlled at a flow rate of 1 mL/ min. The UV/ VIS detector was set at a wavelength of 207 nm. The sample injection volume was 20 μL.
Ultimate 3000 hplc
Manufactured by Phenomenex
Sourced in United States
The Ultimate 3000 HPLC is a high-performance liquid chromatography system designed for efficient and reliable separation, identification, and quantification of a wide range of analytes. It features advanced technology and robust components to deliver consistent and reproducible results.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using ultimate 3000 hplc
1
Quantification of Acetaminophen in Tablets
2
HPLC Analysis of Compound Mixtures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The samples were analyzed by HPLC (UltiMate 3000 HPLC, Phenomenex EnviroSep-PP (125 mm× 4.6 mm) column) using the following conditions: column temperature 30 °C; injection volume 10 μL, mobile phase, acetonitrile, and water; gradient elution program, Table S1 ; UV detection, 254 nm; and fluorescence-detector wavelength program (Tables S2 and S3 ).
3
HeV Nucleoprotein Purification and MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Purified HeV NFL and HeV NCORE were desalted by reverse-phase HPLC (Dionex UltiMate 3000 HPLC) using a C18 Jupiter column (Phenomenex), and the proteins eluted directly onto a microTOF-QII electrospray ionization mass spectrometer (Bruker) using a gradient of acetonitrile containing 0.1% formic acid. The mass of the resulting peaks were determined by maximum entropy-based deconvolution algorithms.
4
Analyzing Vicine and Convicine in BPC Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Vicine and convicine content of the BPI used in the present study and the BPC (Netszch GmbH, Selb Bavaria, Germany) used previously (De Santis et al., 2015a, b) were assessed. The analysis was carried out using an HPLC-based method (NIAB-TAG Ltd, Cambridge, UK), according to Khamassi et al. (2013) , a modification of Lattanzio et al., 1982) . For each BPC sample, 0.5 g samples were extracted in sterile distilled water by vortexing and treating in an ultrasonic water bath at 40 °C for 30 min. After filtering (Whatman No. 1), the filtrate was diluted to 100 mL with sterile distilled water and an aliquot filtered using a 0.45 µm syringe disc-filter and separated on an HPLC system (Dionex Ultimate 3000 HPLC), equipped with a Phenomenex Sphereclone ODS II column (250 x 4.6 mm x 5 µm) with sterile distilled water as the mobile phase at a flow rate of 1.5 mL min -1 and eluent monitored with a diode array detector recording at 229 nm, 254 nm, 280 nm and 400 nm. Peaks were identified as vicine and convicine by their retention time relative to L-DOPA (L-dihydroxyphenylalanine) reference solutions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!