After mice were sacrificed, plasma in each group was collected. The levels of OVA-specific IgE (MBS702947, MyBioSource, Inc., CA, USA), TNF-α (ab208348, Abcam, San Francisco, CA, USA), IL-4 (ab221833, Abcam, San Francisco, CA, USA) and IL-5 (ab204523, Abcam, San Francisco, CA, USA) in the plasma were determined by ELISA kit.
Ab204523
Manufactured by Abcam
Sourced in United States, United Kingdom
Ab204523 is a laboratory reagent for the detection and quantification of a target protein or analyte. The product is designed for use in various experimental techniques, such as immunoassays, but its core function is to provide a reliable and specific detection mechanism. No further details about the intended use or application of this product are provided.
Automatically generated - may contain errors
Lab products found in correlation
Ab208348, by Abcam (2 mentions)
Ab100710, by Abcam (2 mentions)
Ab221833, by Abcam (1 mentions)
Ab119531, by Abcam (1 mentions)
Ab282874, by Abcam (1 mentions)
Ab217608, by Abcam (1 mentions)
Ab215539, by Abcam (1 mentions)
Ab216165, by Abcam (1 mentions)
Ab255729, by Abcam (1 mentions)
Ab185986, by Abcam (1 mentions)
Ab199081, by Abcam (1 mentions)
Ab216167, by Abcam (1 mentions)
4 protocols using ab204523
1
Quantification of Allergy Biomarkers
2
Cytokine Profiling of NALF Samples
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Specimens of NALF were removed from the refrigerator at −70 °C and dissolved at room temperature. Subsequently, the levels of IL-12, interferon-γ (IFN-γ), TNF-α, interleukin-4 (IL-4) and IL-5 were measured with ELISA kits (ab282874, ab119531, ab208348, ab100710 and ab204523, respectively) brought from Abcam (Cambridge, UK), according to the manufacturer's instructions. The absorbance at 450 nm was measured with a microplate reader.
3
Serum IgE and BALF Cytokine Levels
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After sacrificing mice by cervical dislocation, blood was collected from the heart. The blood samples were centrifuged at 3,000 × g for 10 min at 4°C to obtain the serum. The serum levels of OVA-specific IgE were assessed using an ELISA kit (cat. no. ab157718; Abcam). The mice were subsequently intubated, the left main bronchus was ligated and the airway lumina of the right lungs were washed three times (0.4, 0.3 and 0.3 ml, respectively) with sterile saline. The BALF was centrifuged (12,000 × g at 4°C for 10 min) and ELISA kits were used to detect the levels of IL-4 (cat. no. 20186; Quanzhou Ruixin Biological Technology Co., Ltd.), IL-5 (cat. no. ab204523; Abcam), IL-13 (cat. no. ab219634; Abcam) and IFN-γ (cat. no. ab100689; Abcam), according to the manufacturer's protocol.
4
Quantifying Cytokine Levels in Blood and BALF
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The concentrations of IL-18 (ab215539, abcam), IL-1β (ab217608, abcam), IL-17A (ab216167, abcam) and IL-10 (ab185986, abcam) in human peripheral blood serum, and IL-4 (ab100710, abcam), IL-5 (ab204523, abcam), IL-18 (ab216165, abcam), IL-1β (ab241673, abcam), IL-17A (ab199081, abcam) and IL-10 (ab255729, abcam) in mouse BALF were determined by ELISA.
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