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Anti ptie2 tyr992

Manufactured by Merck Group
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Anti-pTie2 (Tyr992) is a laboratory reagent that recognizes the phosphorylated form of the Tie2 protein at the tyrosine 992 residue. It is used in research applications to detect and quantify the activated state of the Tie2 receptor.

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Lab products found in correlation

Anti α actinin, by Merck Group (2 mentions) Axioscan, by Zeiss (2 mentions) Anti mef2c, by Abcam (2 mentions) Anti angpt4, by Thermo Fisher Scientific (2 mentions) Alexa fluor 647 goat anti chicken igy h l, by Abcam (2 mentions) Anti p erk, by Cell Signaling Technology (2 mentions) Anti brdu, by Merck Group (2 mentions) Anti ki67, by Abcam (2 mentions) Alexa fluor 488 goat anti mouse igg, by Thermo Fisher Scientific (2 mentions) Alexa fluor 594 goat anti rabbit igg, by Thermo Fisher Scientific (2 mentions) Anti ctnt, by Abcam (2 mentions) Anti egfp, by Abcam (2 mentions) Anti aurora b, by Abcam (2 mentions) Anti cd31, by Abcam (2 mentions)

2 protocols using anti ptie2 tyr992

1

Immunofluorescence Staining of Cardiac Markers

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Immunofluorescence staining was performed on cryosections as previously described (Han et al., 2014 (link)). The primary antibodies used in this study were: anti-EGFP (Abcam), anti-pERK (Cell Signaling Technology), anti-pTie2 (Tyr992) (Millipore), anti-Mef2c (Abcam), anti-MF20 (DSHB), anti-BrdU (Sigma), anti-α-actinin (Sigma), anti-CD31(Abcam), anti-Angpt4 (Invitrogen), anti cTnT (Abcam), anti-Ki67 (Abcam), and anti-Aurora B (Abcam). The secondary antibodies used in this study were: Alexa Fluor 488 goat anti-mouse IgG (Invitrogen), Alexa Fluor 594 goat anti-rabbit IgG (Invitrogen), Alexa Fluor 647 goat anti-chicken IgY H&L (Abcam). Nikon A1 confocal microscope and Zeiss Axio Scan were used to observe and record the immunostaining images. To quantify the pERK and pTie2 signals, heart area and pERK/pTie2 positive area were analyzed using Surface function in Imaris, then we calculated the ratio of pERK/pTie2 positive area/heart section area.
Wu Z., Shi Y., Cui Y., Xing X., Zhang L., Liu D., Zhang Y., Dong J., Jin L., Pang M., Xiao R.P., Zhu Z., Xiong J.W., Tong X., Zhang Y., Wang S., Tang F, & Zhang B. (2022). Single-cell analysis reveals an Angpt4-initiated EPDC-EC-CM cellular coordination cascade during heart regeneration. Protein & Cell, 14(5), 350-368.
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2

Immunofluorescence Staining of Cardiac Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence staining was performed on cryosections as previously described (Han et al., 2014 (link)). The primary antibodies used in this study were: anti-EGFP (Abcam), anti-pERK (Cell Signaling Technology), anti-pTie2 (Tyr992) (Millipore), anti-Mef2c (Abcam), anti-MF20 (DSHB), anti-BrdU (Sigma), anti-α-actinin (Sigma), anti-CD31(Abcam), anti-Angpt4 (Invitrogen), anti cTnT (Abcam), anti-Ki67 (Abcam), and anti-Aurora B (Abcam). The secondary antibodies used in this study were: Alexa Fluor 488 goat anti-mouse IgG (Invitrogen), Alexa Fluor 594 goat anti-rabbit IgG (Invitrogen), Alexa Fluor 647 goat anti-chicken IgY H&L (Abcam). Nikon A1 confocal microscope and Zeiss Axio Scan were used to observe and record the immunostaining images. To quantify the pERK and pTie2 signals, heart area and pERK/pTie2 positive area were analyzed using Surface function in Imaris, then we calculated the ratio of pERK/pTie2 positive area/heart section area.
Wu Z., Shi Y., Cui Y., Xing X., Zhang L., Liu D., Zhang Y., Dong J., Jin L., Pang M., Xiao R.P., Zhu Z., Xiong J.W., Tong X., Zhang Y., Wang S., Tang F, & Zhang B. (2022). Single-cell analysis reveals an Angpt4-initiated EPDC-EC-CM cellular coordination cascade during heart regeneration. Protein & Cell, 14(5), 350-368.
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