Dab reagent kit

Manufactured by Vector Laboratories

The DAB reagent kit is a laboratory product designed for use in immunohistochemistry applications. It provides the necessary reagents for the detection and visualization of target antigens in tissue samples. The kit includes a diaminobenzidine (DAB) chromogen, which reacts with the enzyme-labeled secondary antibody to produce a brown color, indicating the presence of the target antigen. The specific details and intended use of the product should be obtained from the manufacturer's documentation.

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Lab products found in correlation

Masson s trichrome staining, by Solarbio (1 mentions) Ix50 microscope, by Olympus (1 mentions)

Close spelling variants of this product

DAB kit (218 citations) DAB reagent (37 citations) DAB reagent (peroxidase substrate Kit, (4 citations) Vectastain Universal and DAB reagent kits (2 citations) DAB substrate reagent kit (2 citations)

3 protocols using dab reagent kit

Immunohistochemical Analysis of Phosphorylated Sp4

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Adult rat brains were cut sagittally on a cryostat at 10 microns, mounted, and fixed in 4% paraformaldehyde. After blocking, tissue was incubated overnight at 4°C with Sp4 pSp4, or pSp4 plus 40μg/mL phosphopeptide followed by a biotin conjugated rabbit secondary. Protein was visualized using the DAB reagent kit (Vector laboratories).

Saia G., Lalonde J., Sun X., Ramos B, & Gill G. (2014). Phosphorylation of the transcription factor Sp4 is reduced by NMDA receptor signaling. Journal of neurochemistry, 129(4), 743-752.

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Immunohistochemical and Extracellular Matrix Staining

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IHC staining was conducted using COL1A1 Ready-To-Use IHC Kit (Proteintech, #KHC0205) and STAT1 Ready-To-Use IHC Kit (Proteintech, # KHC1036) according to the kit instructions. After deparaffinization and hydration, the tissue sections were sequentially treated in an antigen retrieval buffer. Primary antibody staining was performed overnight at 4 °C. These slides were then subjected to secondary antibodies for 1 h. For detection, we used the DAB Reagent kit (Vector Laboratories). For IHC staining of Timp1 and Tgfβ1, antibodies against Timp1 (Proteintech, No. 16644-1-AP) and Tgfβ1 (Proteintech, No. 21898-1-AP) were used as primary antibodies.
To visualize the deposited extracellular matrix, Masson’s trichrome staining was performed (Solarbio, Beijing, China, #G1340). Briefly, sections were soaked in Bouin’s solution overnight. The slides were then stained with Weigert’s hematoxylin for 5 min after being rinsed for 10 min under running water. The slides were then rinsed, stained with scarlet-acid fuchsin for 5 min, and then rinsed again. After that, for a total of 5 min, the slides were stained with phosphotungstic/phosphomolybdic, aniline blue, and 2% acetic acid. The slides were then polished, dried, and mounted.

Zhang H., Xia T., Xia Z., Zhou H., Li Z., Wang W., Zhai X, & Jin B. (2024). KIF18A inactivates hepatic stellate cells and alleviates liver fibrosis through the TTC3/Akt/mTOR pathway. Cellular and Molecular Life Sciences: CMLS, 81(1), 96.

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Histological Analysis of Calcification

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GFFP specimens were embedded and frozen in OCT (optimal cutting temperature) media, cut into 5 pm thick sections and mounted on polylysine-coated glass slides. Von Kossa staining was used to evaluate the presence of calcific deposits. Briefly, after washing off OCT with copious amounts of water, a 5% (w/v) AgNO₃ solution was placed on the sections and incubated for 1 hour in front of a 60-watt lamp. Thereafter the samples were exposed to a 5% sodium thiosulfate solution in water for 5 min, washed and mounted in prolong gold anti-fade mounting media and cover-slipped.
Immunohistochemistry (IHC) of adjacent sections was performed to localize the presence of DDR2-Fc. Briefly, after washing off OCT, the sections were blocked with 2% BSA in PBS solution for 1 hr at room temperature and, thereafter, incubated with anti-Fc primary antibody (1 μg/ml) for 2 hrs at room temperature. The sections were then washed and incubated with HRP-conjugated secondary antibody (1 μg /ml) followed by development with a DAB reagent kit (Vector Laboratories). The samples were mounted and cover-slipped and the slides were imaged using an Olympus IX50 microscope using a 20X objective and a Qcolor 3 color camera.

Farzadi A., Renner T., Calomeni E.P., Presley K.F., Karn N., Lannutti J., Dasi L.P, & Agarwal G. (2019). Modulation of biomimetic mineralization of collagen by soluble ectodomain of discoidin domain receptor 2. Materials science & engineering. C, Materials for biological applications, 104, 109905.

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