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2 protocols using trimethoxypropane

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Antioxidant Enzyme Assays and Markers

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Xanthine, nitro blue tetrazolium (NBT), 3,3′-diaminobenzidine, bovine serum albumin, Xanthine oxidase, nicotinamide adenine dinucleotide phosphate (NADPH), oxidized glutathione (GSSG), reduced glutathione (GSH), trimethoxypropane, 5,5-dimethyl-1-pyrrolin-N-oxide (DMPO), hypoXanthine, glutathione reductase (GR), and 2,4-dinitrophenylhydrazine, 1-chloro-2,4-dinitrobenzene (CDNB) were purchased from Sigma (St. Louis, MO, USA). Ethylenediaminetetraacetic acid disodium salt (EDTA), ammonium sulfate, and copper chloride were purchased from JT Baker (Mexico City, Mexico). Hydrogen peroxide (H2O2) formaldehyde, and sodium carbonate were purchased from Mallinckrodt (Paris, KY, USA). Sodium azide was purchased from Merck (Mexico City, Mexico). Trizol reagent, molecular weight markers, agarose, ethidium bromide solution, and synthesized primers (endogenous and antioxidant enzymes) were purchased from Invitrogen (Carlsbad, CA, USA) and the KAPA SYBR FAST qPCR Master Mix kit was purchased from Kappa Biosystems (Kappa Biosystems, London, UK).
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2

Protein Quantification Assay Protocol

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Streptozotocin, bovine serum albumin, bicinchoninic acid, CuSO4, trimethoxypropane, methanesulfonic acid, HCl, FeCl3, 1-methyl-2-phenyl indole, acetonitrile, methanol, acetone, dichloromethane were obtained from Sigma Aldrich Co. (St. Louis, MO, USA). All other reagents were obtained from commercial sources.
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