Poly(acrylic acid)-functionalized magnetic nanoparticles (MNPs, 100 nm hydrodynamic diameter) were purchased from Chemicell GmbH Inc. (Berlin, Germany). Equine heart Mb, H2O2, diammonium salt of 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) [ABTS], tert-Butyl hydroperoxide (t-BuOOH), toluidine blue O (TBO), 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC), and N-hydroxysuccinimide (NHS) were purchased from Sigma. High purity graphite disk electrodes (HPGs, geometric area 0.2 cm2) were purchased from McMaster-Carr (Atlanta, GA, USA). All other chemicals were of high purity analytical grade.
Diammonium salt of 2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid
Manufactured by Merck Group
Sourced in United States
Diammonium salt of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) is a chemical compound used as a chromogenic substrate in various biochemical and analytical applications. It serves as an indicator for the detection and quantification of enzymatic activities.
Automatically generated - may contain errors
Lab products found in correlation
Trichloroacetic acid, by Merck Group (3 mentions)
Ferric chloride, by Merck Group (2 mentions)
Folin ciocalteu phenol reagent, by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Sodium carbonate, by Merck Group (2 mentions)
Potassium ferricyanide, by Merck Group (2 mentions)
Gallic acid, by Merck Group (2 mentions)
L ascorbic acid, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Ethanol, by Merck Group (2 mentions)
Potassium persulfate, by Merck Group (2 mentions)
N hydroxysuccinimide nhs, by Merck Group (1 mentions)
1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride edc, by Merck Group (1 mentions)
Boron trifluoride methanol, by Merck Group (1 mentions)
N hexane, by Thomas Scientific (1 mentions)
Anhydrous sodium sulfate, by Merck Group (1 mentions)
Sulfuric acid, by Merck Group (1 mentions)
Oleic acid, by Merck Group (1 mentions)
Linoleic acid, by Merck Group (1 mentions)
Palmitic acid, by Merck Group (1 mentions)
5 protocols using diammonium salt of 2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid
1
Functionalized Magnetic Nanoparticle-Based Biosensing
2
Comprehensive Analytical Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All the chemicals and reagents used in this study were of analytical grade and were used without further purification. Water and n-hexane were purchased from Thomas Scientific (Philadelphia, PA, USA) and Fisher Scientific (Pittsburgh, PA, USA), respectively. All the other chemicals and reagents including methanol, anhydrous sodium sulfate (Na2SO4), sulfuric acid (H2SO4), boron trifluoride-methanol (BF3-MeOH) solution, sodium hydroxide (NaOH), gallic acid, Folin-Ciocalteu phenol reagent, L-ascorbic acid, sodium carbonate, potassium ferricyanide, trichloroacetic acid, ferric chloride, diammonium salt of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), and standards of palmitic acid, stearic acid, oleic acid, linoleic acid, and linolenic acid were obtained from Sigma Aldrich (St. Louis, MO, USA).
3
Phytochemical Extraction and Antioxidant Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Folin–Ciocalteu’s phenol reagent, methanol, ethanol, glacial acetic acid, formalin, potassium chloride, sodium acetate, sodium carbonate (Na2CO3), potassium persulfate, hydrochloric acid, and ferric(III) chloride were purchased from Merck (Darmstadt, Germany). The diammonium salt of 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), gallic acid and 2,2-diphenyl-1-picrylhydrazyl (DPPH), 3,3′-diaminobenzidine (DAB), p-hydroxybenzoic acid, vanillic acid, 3,4-dimethoxyphenol, chlorogenic acid, floroglucinol, quercetin 3-rutinoside, and quercetin were purchased from Sigma-Aldrich (Saint Louis, MO, USA).
4
Quantitative Analysis of Anthocyanins and Isoflavones
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All the chemicals and reagents used in the present study were of analytical grade (purity > 98.9%). Anthocyanin reference standards including cyanidin-3-O-glucoside, cyanidin-3-O-galactoside, peonidin-3-O-glucoside, and malvidin-3-O-glucoside were purchased from PhytoLab (Bavaria, Germany). Isoflavone standards including malonyldaidzin, malonylgenistin acetylgenistin, acetylglycitin, and acetyldaidzin were purchased from Synthose (Ontario, Canada) while malonylglycitin was purchased from Fujifilm (Osaka, Japan). HPLC-grade solvents including water and methanol were purchased from Thermo Fisher (Swedesboro, NJ, USA). Acetonitrile and acetic acid were purchased from Honeywell (Charlotte, NC, USA) and Merck (Darmstadt, Germany), respectively. The remaining reference standards and reagents including delphinidin-3-O-glucoside, petunidin-3-O-glucoside, daidzein, daidzin, genistein, genistin, glycitein, glycitin, hydrochloric acid (HCl), acetone, ethanol, formic acid, gallic acid, Folin-Ciocalteu phenol reagent, L-ascorbic acid, sodium carbonate, potassium ferricyanide, trichloroacetic acid, ferric chloride, diammonium salt of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 1,1-diphenyl-2-picrylhydrazyl radical (DPPH∙), and 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) were purchased from Sigma Aldrich (St.Louis, MO, USA).
5
Antioxidant Activity of Rice Bran Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
γ-Oryzanol was purchased from Oryza Oil & Fat Chemical Co., Ltd. (Ichinomiya, Japan). Tocopherol and tocotrienol were obtained from Merck KGaA (Darmstadt, Germany). The individual polycosanol standards, 5α-cholestane, β-sitosterol, campesterol, stigmasterol, diammonium salt of 1,1-diphenyl-2-picrylhydrazyl (DPPH), diammonium salt of 2,2-azino-bis-(3-ethylbenzothiazoline-6- sulfonic acid) (ABTS), ethylene diamine tetra acetic acid (EDTA), dimethyl sulfoxide (DMSO), 5,5’-dithiobis-2-nitrobenzoic acid (DTNB), 2’,7’-dichlorofluorescein diacetate (DCFH-DA), glutathione reductase (GR), reduced glutathione (GSH), tert-butyl hydroperoxide (TBHP), potassium ferricyanide, potassium persulfate, and trichloroacetic acid were purchased from Sigma Chemical Co. (St. Louis, MO, USA).
HepG2 cells were obtained from the Korean Collection for Type Cultures (Daejeon, Korea). Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin, and trypsin-EDTA were purchased from Gibco BRL (Gaithersburg, MD, USA). Rice bran provided by the Rural Development Administration of Korea (Wanju, Korea) was heated at 100°C for 30 min.
HepG2 cells were obtained from the Korean Collection for Type Cultures (Daejeon, Korea). Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin, and trypsin-EDTA were purchased from Gibco BRL (Gaithersburg, MD, USA). Rice bran provided by the Rural Development Administration of Korea (Wanju, Korea) was heated at 100°C for 30 min.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!