The serum was dissolved at room temperature and mixed well. The cell culture supernatant of HaCAT cells after indicated treatment was obtained after centrifugation (1000 r/min, 10 min). The levels of IFN-γ, IL-8 and IL-6 in the mice serum were detected by Mouse IFN-γ ELISA Kit (Beyotime), Mouse IL-8 ELISA Kit (Wuhan Saipei Biotechnology Co., Ltd) and Mouse IL-6 ELISA Kit (Beyotime) and the levels of IFN-γ, IL-8 and IL-6 in the culture supernatant of HaCAT cells were detected by Human ELISA Kits (Beyotime).
Mouse il 6 elisa kit
Manufactured by Beyotime
Sourced in China, United States
The Mouse IL-6 ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of mouse interleukin-6 (IL-6) levels in cell culture supernatants, serum, and plasma samples.
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Lab products found in correlation
Mouse tnf α elisa kit, by Beyotime (8 mentions)
Mouse il 1β elisa kit, by Beyotime (6 mentions)
Mouse il 18 elisa kit, by Beyotime (2 mentions)
Microplate reader, by Thermo Fisher Scientific (2 mentions)
Mouse ifn γ elisa kit, by Beyotime (1 mentions)
Human tnf α elisa kit, by Beyotime (1 mentions)
Human il 6 elisa kit, by Beyotime (1 mentions)
Human il 1β elisa kit, by Beyotime (1 mentions)
Powerup sybr green, by Thermo Fisher Scientific (1 mentions)
Sds page protein loading buffer, by Beyotime (1 mentions)
D galactose d gal, by Merck Group (1 mentions)
Mouse lbp elisa kit, by Abcam (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Bis gma, by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Biosharp (1 mentions)
Dcfh da, by MedChemExpress (1 mentions)
Cell counting kit 8 cck 8, by Beyotime (1 mentions)
Ldh assay kit, by Beyotime (1 mentions)
Dulbecco s modified eagle medium dmem, by Keygen Biotech (1 mentions)
21 protocols using mouse il 6 elisa kit
1
Cytokine Quantification in Cell Culture
2
Cytokine Levels in Lung Lavage
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Take 2 mL of lung lavage solution from each group of mice, centrifuge at 710 rcf for 5 min with a centrifugal radius of 10 cm, and collect the supernatant. Detect the levels of IL-6(Beyotime, Mouse IL-6 ELISA Kit), and TNF-a(Beyotime, Mouse TNF-α ELISA Kit) according to the instructions of the kit. Add 100 μl working solution to the corresponding wells of the plate, incubate for 1.5 h, remove the liquid from the plate and add 100 μl biotinylated antibody working solution, incubate for 1 h, remove the liquid from the plate and wash with PBS. Then add 100 μl HRP conjugate working solution, incubate for 0.5 h, remove the liquid from the plate, wash with PBS, add 90 μl substrate solution, and after 15 min, add 50 μl stop solution. Detect the OD value at 400 nm wavelength and calculate the levels of IL-6, and TNF-a.
3
Cytokine Profiling in Kidney Cells
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The concentrations of interleukin- (IL-) 1β, IL-6, and tumor necrosis factor- (TNF-) α in HK-2 cells were detected via the Human IL-1β ELISA Kit (PI305, Beyotime), Human IL-6 ELISA Kit (PI330, Beyotime), and Human TNF-α ELISA Kit (PT518, Beyotime), respectively. The concentrations of IL-1β, IL-6, and TNF-α in kidney tissues were measured via the Mouse IL-1β ELISA Kit (PI301, Beyotime), Mouse IL-6 ELISA Kit (PI326, Beyotime), and Mouse TNF-α ELISA Kit (PT512, Beyotime), respectively.
4
Nobiletin Modulates D-Galactose-Induced Inflammation
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Nobiletin (Nob, IN0210) was obtained from MedChemExpress (Beijing, China). Nob was prepared as a stock solution with DMSO, dispense and kept at −80 °C and use up within six months. D-galactose (D-gal, G5388) was purchased from Sigma-Aldrich (St. Louis, MO, USA). RIPA Lysis Buffer (P0013B), Phenylmethanesulfonyl fluoride (PMSF, ST506), 5× SDS-PAGE protein loading buffer (P0015L), BCA Protein Assay Kit (P0012), Mouse TNF-α ELISA Kit (PT512), and Mouse IL-6 ELISA Kit (PI326) were purchased from Beyotime Biotechnology Company (Shanghai, China). cDNA synthesis kit (G592) was obtained from abm (Vancouver, Canada). PowerUp™ SYBR™ Green (A25742) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Mouse LBP ELISA Kit (ab269542) was obtained from abcam (Shanghai, China). The primers were purchased from Beijing Tianyi Huiyuan Biotechnology Co., Ltd. (Beinjing, China). p-Ser2448-mTOR (5536T), p-p70 S6 Kinase Antibody (9234S) and p70 S6 Kinase Antibody (9202S) were purchased from Cell Signaling Technology. Furthermore, mToR (28273-1-AP) and GAPDH Polyclonal antibody (10494-1-AP) were obtained from Proteintech.
5
Cytokine and FNDC5 Levels in Heart
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TNF-α, IL-1β levels in heart tissues were measured with mouse TNF-α and IL-1β ELISA Kits (Excell Bio, Shanghai, China). IL-6 levels in heart tissues were measured with mouse IL-6 ELISA Kit (Beyotime Biotechnology, Shanghai, China). ELISA kit for FNDC5 was obtained from Phoenix pharmaceuticals (EK-067-19, Burlingame, USA).
6
Serum Biomarkers for Metabolic Health
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The content of triglyceride, low density lipoprotein cholesterol (LDL-C), and LPS in serum were determined using enzyme-linked immunosorbent assay kit (Cloud Clone Corp. Product Number: SEB006Mu, SEB107Mu and SEB526Ge). Serum concentration of IL-6 was measured with mouse IL-6 ELISA kit (Beyotime Biotechnology Co., Ltd., Product Number: PI326). The serum fasting insulin (FINS) content was detected using mouse FINS ELISA kit (Jiangsu Cote Biological Technology Co. Ltd., Product Number: KT2579). The homeostasis model assessment-insulin resistance (HOMA-IR) index was calculated with the formula: (FBG value × FINS value)/22.5, and the HOMA-β index was calculated with the formula: 20 × FINS value/(FBG value - 3.5).
7
Cytokine Quantification in Cell Samples
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Cell culture supernatants and lung tissues were collected and frozen at −80 °C. Cytokines were quantified by specific ELISA kits following the manufacturer’s instructions. Human IL-1β (DKW12-3012/-2012), human IL-6 (DKW12-1060/-2060) and human TNF-α (DKW12-1720/-2720) ELISA Kits were purchased from Dakewe Biological Technology Co., Ltd. (Beijing, China). Mouse IL-1β ELISA Kit (PI301), mouse IL-6 ELISA Kit (PI326) and mouse IL-TNFα ELISA Kit (PT512) were purchased from Beyotime Institute of Biotechnology (Shanghai, China). Mouse IL-8 ELISA Kit (MBS261967) were purchased from MyBioSource Bio Co., Ltd. (Vancouver, BC, Canada).
8
Fiberglass-Reinforced Dental Resin Composite
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High-strength fiberglass (diameter: 11 mm, Sinoma Technology Co., Ltd, China), resin matrix including bisphenol A-glycidyl methacrylate (Bis-GMA, AR, Sigma-Aldrich, City of Saint Louis, USA) and Methyl methacrylate (MMA; AR, Shanghai Titan Chemical Co., Ltd, China), a photoinitiator system including camphorquinone (CQ, Shanghai Chemical Technology Co., Ltd, China) and dimethylaminoethyl methacrylate (DMAEMA; AR, Shanghai Titan Chemical Co., Ltd, China), HCl (AR, Liyang Dongfang Chemical Reagent Co., Ltd, China), and 3-(trimethoxysilyl)propyl methacrylate (KH570, AR, Sigma-Aldrich, USA) were used without purification, chemically curing methyl methacrylate resin (New Century Dental, China), single bond universal (3M, America), dulbecco’s modified eagle medium (DMEM, KeyGEN BioTECH, China), Cell Counting Kit-8 (CCK-8; Beyotime Biotechnology Co., Ltd., Shanghai, China), LDH Assay Kit (Beyotime Biotechnology Co., Ltd., Shanghai, China), DCFH-DA (MedChemExpress, State of New Jersey, USA), Mouse IL-6 ELISA kit (Beyotime Biotechnology Co., Ltd., Shanghai, China), 4% paraformaldehyde (Biosharp, Anhui, China), phalloidine (APExBIO, Houston, USA), 4’,6-diamidino-2-phenylindole (DAPI; APExBIO, Houston, USA).
9
Evaluation of Inflammatory Cytokines
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Serum levels of TNF-α (Mouse TNF-α ELISA kit; cat no. PT512) and IL-6 (Mouse IL-6 ELISA kit; cat no. PI326) in D-GalN/LPS-treated mice, and those in the supernatant (centrifugation at 500 × g at 4°C for 5 min) of BNLCL2 cells were determined by ELISA kits (Beyotime Institute of Biotechnology) according to the manufacturer's instructions.
10
ELISA Quantification of IL-6 and TNF-α
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The supernatant from chondrocyte culture medium or mouse serum was collected. The IL-6 (Mouse IL-6 ELISA Kit, PI326, Beyotime) and tumor necrosis factor-α (TNF-α; Mouse TNF-α ELISA Kit, PT512, Beyotime) levels were detected, respectively, using the ELISA kits.
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