Tunicamycin, chloroquine, N-acetyl cysteine (NAC), DETA-NONOate, mammalian cell lysis kit, Rabbit anti-actin antibody, DMEM:F12 medium, Mission esiRNA for SQSTM1, and eNOS siRNA were purchased from Sigma-Aldrich. Glycan Differentiation kit, WST-1 cell viability kit, and X-tremeGENE HP DNA Transfection reagent were obtained from Roche. ROS detection kit, gold antifade mounting medium, NuPAGE Novex 4-12% Bis-Tris gels, goat anti-rabbit IgG-alexa 488, Goat-anti-rabbit IgG-Alexa Fluor 568, First Strand cDNA synthesis kit, Fetal Bovine Serum were from Invitrogen. JC1 mitochondrial membrane potential assay kit was from Cayman. Apodirect TUNEL assay kit was purchased from Millipore. Ultralow attachment tissue culture plate was from Nunc. TurboFect siRNA Transfection Reagent, DAPI, and HRP chemiluminescence substrate were from Thermo Scientific. Anti-ubiquitin antibody (clone P4D1) was from Biolegend. Anti rabbit antibodies against LC3, p62, and calnexin, Rabbit mAb phosphor-p70 S6 Kinase (Thr 389), mouse anti-eNOS mAb were obtained from Cell signaling Technology. pcDNA3-eNOS-GFP was obtained from Addgene. LC3-GFP and p62-EGFP constructs were kind gifts from Dr. Beth Levine (Columbia University, NY) and Dr. Terje Johansen (University of Tromso, Norway), respectively.
Wst 1 cell viability kit
Manufactured by Roche
The WST-1 cell viability kit is a colorimetric assay used to quantify cell proliferation and cell viability. The kit utilizes the WST-1 reagent, which is reduced by cellular enzymes to produce a colored formazan dye. The amount of formazan dye generated is directly proportional to the number of metabolically active cells in the sample. The kit provides a simple and reliable method for measuring cell populations.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)
Antifade gold mounting medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Alexa fluor 568 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Mammalian cell lysis kit, by Merck Group (1 mentions)
Rabbit anti actin antibody, by Merck Group (1 mentions)
Enos sirna, by Merck Group (1 mentions)
First strand cdna synthesis kit, by Thermo Fisher Scientific (1 mentions)
Alexa 488 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Deta nonoate, by Merck Group (1 mentions)
Apodirect tunel assay kit, by Merck Group (1 mentions)
Turbofect sirna transfection reagent, by Thermo Fisher Scientific (1 mentions)
Dmem f12 medium, by Merck Group (1 mentions)
Nupage novex 4 12 bis tris gel, by Thermo Fisher Scientific (1 mentions)
Jc 1 mitochondrial membrane potential assay kit, by Cayman Chemical (1 mentions)
Ros detection kit, by Thermo Fisher Scientific (1 mentions)
Tunicamycin, by Merck Group (1 mentions)
X tremegene hp dna transfection reagent, by Roche (1 mentions)
Hacat cells, by American Type Culture Collection (1 mentions)
2 protocols using wst 1 cell viability kit
1
Autophagy regulation by eNOS signaling
2
HaCaT Cell Viability and Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HaCaT cells were purchased from American Type Culture Collection (ATCC, Rockville, MD, USA). The WST-1 cell viability kit was purchased from Roche Applied Science. A 5-bromo-2′-bromodeoxyuridine (BrdU) cell proliferation assay kit was purchased from cell. Poly I-C was used as a TLR3-stimulating reagent. This study was approved by the Institutional Review Board of the Uijeongbu St. Mary's Hospital (UC18EESI0007).
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